Cloning and Expression Analysis of the Anterior ParaHox Genes, Gsh1 and Gsh2 From Xenopus tropicalis
Cloning and Expression Analysis of the Anterior ParaHox Genes, Gsh1 and Gsh2 From Xenopus tropicalis
复制标题
DOI：
10.1002/dvdy.21816
复制
发表时间：
2009-01-01
影响因子：
2.5
通讯作者：
Isaacs, Harry V.
中科院分区：
文献类型：
--
作者：
Illes, Jean C.;Winterbottom, Emily;Isaacs, Harry V.
Gsx类蛋白是ParaHox同源域转录因子家族的成员，在神经系统的特化和模式化中具有保守的作用。我们报告的克隆两个GSX基因，GSH 1和GSH 2，从青蛙热带爪蟾。我们证明了存在一个单一的，完整的爪蟾ParaHox集群，含有GSH 1，Pdx和Cdx 2，加上三个退化集群含有GSH 2，Cdx 1和Cdx 4。来自完整ParaHox簇的基因的前表达边界与其基因组组织是共线性的。我们发现，GSH 1和GSH 2表现出复杂的，重叠的模式内的前神经系统从开放的神经板阶段的表达。我们还发现，Gsh 2，Nkx 6，Msx 1跨中-横轴的两栖动物神经板的表达是惊人的相似，在果蝇神经外胚层的相关基因。这些研究结果提供了进一步的证据，一个保守的途径调节背腹图案的两侧。Developmental Dynamics 238：194-203，2009. (C)2008 Wiley-Liss，Inc.
Gsx class proteins are members of the ParaHox homeodomain transcription factor family with conserved roles in specification and patterning of the nervous system. We report the cloning of two Gsx genes, Gsh1 and Gsh2, from the frog Xenopus tropicalis. We demonstrate the existence of a single, intact Xenopus ParaHox cluster, containing Gsh1, Pdx, and Cdx2, plus three degenerate clusters containing Gsh2, Cdx1, and Cdx4. Anterior expression boundaries of genes from the intact ParaHox cluster are co-linear with respect to their genomic organization. We show that Gsh1 and Gsh2 exhibit complex, overlapping patterns of expression within the anterior nervous system from open neural plate stages. We also find that expression of Gsh2, Nkx6, and Msx1 across the medio-lateral axis of the amphibian neural plate is strikingly similar to that of related genes in the Drosophila neuroectoderm. These findings provide further evidence for a conserved pathway regulating dorso-ventral patterning in the Bilateria. Developmental Dynamics 238: 194-203, 2009. (C) 2008 Wiley-Liss, Inc.