Molecular cloning and characterization of the mouse CGT gene encoding UDP-galactose ceramide-galactoysyltransferase (cerebroside synthetase)
Molecular cloning and characterization of the mouse CGT gene encoding UDP-galactose ceramide-galactoysyltransferase (cerebroside synthetase)
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DOI：
10.1006/geno.1996.0342
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发表时间：
1996-07-01
期刊：
影响因子：
4.4
通讯作者：
Stoffel, W
中科院分区：
文献类型：
--
作者：
Bosio, A;Binczek, E;Stoffel, W
UDP-半乳糖神经酰胺半乳糖转移酶，CGT，EC 2.4.1.45，是脑苷脂和硫脂生物合成的关键酶，脑苷脂和硫脂是中枢神经系统和周围神经系统髓鞘中含量最丰富的糖鞘糖脂。CGT基因的细胞特异性和高度时间调控的表达被认为在少突胶质细胞和雪旺细胞的分化中起着重要作用。分离并鉴定了3个编码小鼠CGT基因的基因组克隆。该基因分布在-gt；42kb上，编码序列分布在5个外显子上，从77到822bp不等。通过引物延伸实验确定可能的转录起始点。CGT基因在进化过程中高度保守。(C)1996年学术出版社。
UDP-galactose ceramide galactosyltransferase, CGT, EC 2.4.1.45, is the key enzyme in the biosynthesis of cerebrosides and sulfatides, which are the most abundant glycosphingolipids in the myelin of the central nervous system and the peripheral nervous system. The cell-specific and highly time-regulated expression of the CGT gene is thought to play an important role in oligodendrocyte and Schwann cell differentiation. Three genomic clones encoding the mouse CGT gene were isolated and characterized. The gene is distributed over >42 kb, and the coding sequence is distributed over five exons ranging from 77 to 822 bp. Putative transcription start sites were determined by primer extension experiments. The CGT gene locus is highly conserved during evolution. (C) 1996 Academic Press, Inc.