Structure and function analysis of LIN-14, a temporal regulator of postembryonic developmental events in Caenorhabditis elegans
Structure and function analysis of LIN-14, a temporal regulator of postembryonic developmental events in Caenorhabditis elegans
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DOI：
10.1128/mcb.20.6.2285-2295.2000
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发表时间：
2000-03-01
影响因子：
5.3
通讯作者：
Ambros, V
中科院分区：
文献类型：
--
作者：
Hong, Y;Lee, RC;Ambros, V
在秀丽隐杆线虫胚后发育过程中，异时基因lin-14控制着不同细胞类型发育事件的发生时间。三个替代林-II转录预测编码亚型的一种新的核蛋白，不同的氨基末端结构域。在本文中，我们报告说，替代氨基末端结构域的LIN-14的外显子9至13内的羧基末端区域是必要的，并足以在体内LIN-14的功能。一个转基因能够表达三个替代lin-14基因产物之一，拯救lin-14无效突变，并在发育上受lin-4调控。这表明，替代性lin-12基因产物的部署对于LIN-14在不同细胞类型中调节下游基因的能力或对于lin-4体内调节LIN-14水平不是关键的。LIN-14的羧基末端区域包含对于LIN-14功能至关重要的不寻常的扩展的核定位结构域。这些结果支持LIN-14控制C.通过调节细胞核中的基因表达来控制线虫。
During postembryonic development of Caenorhabditis elegans, the heterochronic gene lin-14 controls the timing of developmental events in diverse cell types. Three alternative lin-II transcripts are predicted to encode isoforms of a novel nuclear protein that differ in their amino-terminal domains. In this paper, we report that the alternative amino-terminal domains of LIN-14 are dispensable and that a carboxy-terminal region within exons 9 to 13 is necessary and sufficient for in vivo LIN-14 function. A transgene capable of expressing only one of the three alternative lin-14 gene products rescues a lin-14 null mutation and is developmentally regulated by lin-4. This shows that the deployment of alternative lin-II gene products is not critical for the ability of LIN-14 to regulate downstream genes in diverse cell types or for the in vivo regulation of LIN-14 level by lin-4, The carboxy-terminal region of LIN-14 contains an unusual expanded nuclear localization domain which is essential for LIN-14 function. These results support the view that LIN-14 controls developmental timing in C. elegans by regulating gene expression in the nucleus.