Thioester-containing proteins regulate the Toll pathway and play a role in Drosophila defence against microbial pathogens and parasitoid wasps.
Thioester-containing proteins regulate the Toll pathway and play a role in Drosophila defence against microbial pathogens and parasitoid wasps.
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DOI：
10.1186/s12915-017-0408-0
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发表时间：
2017-09-05
期刊：
影响因子：
5.4
通讯作者：
Lemaitre B
中科院分区：
文献类型：
--
作者：
Dostálová A;Rommelaere S;Poidevin M;Lemaitre B
含硫酯蛋白(TEP)家族的成员对昆虫和哺乳动物的宿主防御都有贡献。然而，它们在果蝇免疫反应中的作用尚不清楚。在这项研究中，我们通过产生一个突变的果蝇品系TEPqΔ来研究TEPs在果蝇免疫中的作用，该品系缺乏四个免疫诱导的TEP1、2、3和4。对TEPqΔ果蝇的生存分析揭示了这些蛋白质在防御昆虫病原真菌、革兰氏阳性细菌和寄生蜂方面的重要性。我们的结果证实了TEP是有效吞噬细菌所必需的，特别是对于测试的两种革兰氏阳性菌，金黄色葡萄球菌和粪肠球菌。此外，我们发现TEPqΔ果蝇在微生物感染时减少了Toll途径的激活，导致抗菌肽基因的表达降低。上位性分析表明，在Toll途径激活的初始阶段，TEP的功能位于丝氨酸蛋白酶MoSP的上游或独立于丝氨酸蛋白酶MoSP。总而言之，我们的研究为TEP在昆虫免疫中的作用带来了新的见解。结果表明，TEP参与果蝇体液免疫反应和细胞免疫反应。特别是，它表明了TEP在防御革兰氏阳性细菌和昆虫病原真菌方面的重要性，特别是通过促进Toll途径的激活。本文的在线版本(doi：10.1186/s12915-0170408-0)包含补充材料，授权用户可以使用。
Members of the thioester-containing protein (TEP) family contribute to host defence in both insects and mammals. However, their role in the immune response of Drosophila is elusive. In this study, we address the role of TEPs in Drosophila immunity by generating a mutant fly line, referred to as TEPq Δ, lacking the four immune-inducible TEPs, TEP1, 2, 3 and 4. Survival analyses with TEPq Δ flies reveal the importance of these proteins in defence against entomopathogenic fungi, Gram-positive bacteria and parasitoid wasps. Our results confirm that TEPs are required for efficient phagocytosis of bacteria, notably for the two Gram-positive species tested, Staphylococcus aureus and Enterococcus faecalis. Furthermore, we show that TEPq Δ flies have reduced Toll pathway activation upon microbial infection, resulting in lower expression of antimicrobial peptide genes. Epistatic analyses suggest that TEPs function upstream or independently of the serine protease ModSP at an initial stage of Toll pathway activation. Collectively, our study brings new insights into the role of TEPs in insect immunity. It reveals that TEPs participate in both humoral and cellular arms of immune response in Drosophila. In particular, it shows the importance of TEPs in defence against Gram-positive bacteria and entomopathogenic fungi, notably by promoting Toll pathway activation. The online version of this article (doi:10.1186/s12915-017-0408-0) contains supplementary material, which is available to authorized users.
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影响因子：
6.7
作者：
Binggeli O;Neyen C;Poidevin M;Lemaitre B
通讯作者：
Lemaitre B
影响因子：
4.5
作者：
Chakrabarti S;Dudzic JP;Li X;Collas EJ;Boquete JP;Lemaitre B
通讯作者：
Lemaitre B
果蝇先天免疫：酚氧化酶原的区域和功能专业化。
影响因子：
5.4
作者：
Dudzic JP;Kondo S;Ueda R;Bergman CM;Lemaitre B
通讯作者：
Lemaitre B
影响因子：
5.3
作者：
Brankatschk, Marko;Eaton, Suzanne
通讯作者：
Eaton, Suzanne
影响因子：
2.1
作者：
Brun, S;Vidal, S;Lemaitre, B
通讯作者：
Lemaitre, B