Bioprospecting of novel and bioactive compounds from marine actinomycetes isolated from South China Sea sediments
Bioprospecting of novel and bioactive compounds from marine actinomycetes isolated from South China Sea sediments
复制标题
从南海沉积物中分离出的海洋放线菌中新型生物活性化合物的生物勘探
DOI：
10.1007/s00284-017-1358-z
复制
发表时间：
2018
影响因子：
2.6
通讯作者：
Song Fuhang
中科院分区：
文献类型：
--
作者：
Yang Na;Song Fuhang
与陆地菌株相比，海洋放线菌作为天然产物的潜在来源的研究较少。目前，对南海沉积物中可培养放线菌的研究较少。本研究采用传统的培养分离方法，从南海沉积物中分离到28株放线菌。16S rRNA基因序列分析表明，这些菌株分属于5个科7个属。十二种代表性菌株至少拥有一种编码聚酮合酶I、II和非核糖体肽合成酶的生物合成基因。其中4株具有抗草分枝杆菌活性，5株具有抗耐甲氧西林金黄色葡萄球菌活性。Salinisporasp. NHF 45、诺卡氏菌属（Nocardiopsis sp.）NHF 48和链霉素P. NHF 86用于发现新的和生物活性的化合物。最后，从海洋诺卡氏菌（Nocardiopsis sp.）NHF 48，一种来自海洋链霉菌的paulomenol类似物。NHF 86和一种新的利福霉素B来源。NHF 45。本研究的结论是，海洋放线菌，我们从中国南海沉积物中分离，将是一个合适的来源，为开发新的和生物活性的化合物。
Marine actinomycetes are less investigated compared to terrestrial strains as potential sources of natural products. To date, few investigations have been performed on culturable actinomycetes associated with South China Sea sediments. In the present study, twenty-eight actinomycetes were recovered from South China Sea sediments after dereplication by traditional culture-dependent method. The 16S rRNA gene sequences analyses revealed that these strains related to five families and seven genera. Twelve representative strains possessed at least one of the biosynthetic genes coding for polyketide synthase I, II, and nonribosomal peptide synthetase. Four strains had anti-Mycobacterium phleiactivities and five strains had activities against methicillin-resistantStaphylococcus aureus. 10 L-scale fermentation of strainsSalinisporasp. NHF45,Nocardiopsissp. NHF48, andStreptomycessp. NHF86 were carried out for novel and bioactive compounds discovery. Finally, we obtained a novel α-pyrone compound from marineNocardiopsissp. NHF48, an analogue of paulomenol from marineStreptomycessp. NHF86 and a new source of rifamycin B, produced bySalinisporasp. NHF45. The present study concluded that marine actinomycetes, which we isolated from South China Sea sediments, will be a suitable source for the development of novel and bioactive compounds.