Using the Enzymatic Growth of Nanoparticles To Create a Biosensor An Undergraduate Quantitative Analysis Experiment
Using the Enzymatic Growth of Nanoparticles To Create a Biosensor An Undergraduate Quantitative Analysis Experiment
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DOI：
10.1021/ed086p712
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发表时间：
2009-06-01
影响因子：
3
通讯作者：
Bishnoi, Sandra Whaley
中科院分区：
文献类型：
--
作者：
Bai, James;Flowers, Kaleyhia;Bishnoi, Sandra Whaley
这个第二年的分析化学实验室让学生接触生物传感器和灵敏度，线性范围和动态范围的概念。金纳米粒子的使用提供了一种过氧化物（第一部分）和葡萄糖（第二部分）传感的方法，可以使用单波长或扫描紫外可见分光光度计进行比色监测。实验的每个部分可以在三小时的实验室时间内进行三次，或者两次合并可以在三小时内进行。
This second-year analytical chemistry laboratory exposes students to biosensors and the concepts of sensitivity, linear range, and dynamic range. The use of gold nanoparticles provides a method of peroxide (Part I) and glucose (Part II) sensing that can be monitored colorimetrically using either a single wavelength or scanning UV–vis spectrophotometer. Each part of the experiment can be conducted in triplicate within a three-hour laboratory period or both combined can be conducted within the three-hour period.