Origin of neointimal cells in arteriovenous fistulae: bone marrow, artery, or the vein itself?
Origin of neointimal cells in arteriovenous fistulae: bone marrow, artery, or the vein itself?
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DOI：
10.1111/j.1525-139x.2011.00870.x
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发表时间：
2011-03
影响因子：
1.6
通讯作者：
Vazquez-Padron RI
中科院分区：
文献类型：
--
作者：
Skartsis N;Manning E;Wei Y;Velazquez OC;Liu ZJ;Goldschmidt-Clermont PJ;Salman LH;Asif A;Vazquez-Padron RI
The exact origin of the neointimal cells in arteriovenous fistulae (AVFs) has not been conclusively established. This study attempts to elucidate the anatomical source of neointimal cells in AVFs. Experimental fistulas were created in Lewis wild type (WT) and transgenic rats that constitutively expressed the Green Fluorescent Protein (GFP) in all tissues. AVFs were created by anastomosing the left renal vein to the abdominal aorta after unilateral nephrectomy. Three potential sources (bone marrow, feeding artery and the fistula vein itself) were examined. The contribution of bone marrow (BM) derived cells to the AVF neointima was examined in lethally irradiated WT rats that had been rescued with GFP BM cells. Neointimal cells in these chimeric rats were mostly GFP negative indicating the non-BM origin of those cells. Then, the contribution of arterial cells to the AVF neointima was assessed in a fistula made with a GFP aorta that had been implanted orthotopically into a WT rat. In this model, most of the neointimal cells were also GFP negative demonstrating that AVF neointimal cells are not derived from the feeding artery cells. Finally to study local resident cells contribution to the formation of neointimal lesions, a composite fistula was created by interposing a GFP vein between the renal vein and the aorta in a WT recipient rat. GFP neointimal cells were only found in the transplanted vein. This study suggests that neointimal cells originate from the local resident cells of the AVF.
