Fgf- and Bmp-signaling regulate gill regeneration in Ambystoma mexicanum
Fgf- and Bmp-signaling regulate gill regeneration in Ambystoma mexicanum
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Fgf 和 Bmp 信号传导调节墨西哥钝口螈的鳃再生
DOI：
10.1016/j.ydbio.2019.04.011
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发表时间：
2019
影响因子：
2.7
通讯作者：
Satoh Akira
中科院分区：
文献类型：
--
作者：
Saito Nanami;Nishimura Koki;Makanae Aki;Satoh Akira
与其他附属物如肢体和尾巴的再生相比，鳃的再生还没有得到很好的研究。本文以美西螈鳃再生为研究对象，发现Fgf-和bmp -信号参与了美西螈鳃再生机制。蝾螈有三对鳃支，每对鳃支都有多条鳃丝。鳃由富含细胞外基质的间质和表皮组成。鳃神经由位于头部的三叉神经节供给。去神经支配没有引起鳃再生反应。神经和鳃表达Fgf和Fgf基因，用Fgf-和bmp -信号抑制剂治疗动物会导致与去神经支配的鳃相似的表型。诱导附属物是两栖动物再生研究中的标准实验。在我们的研究中，侧边损伤可以诱导副鳃，这表明细轴突纤维和间充质fgfs和bmps有助于诱导副鳃结构。这种副鳃诱导受到去神经支配的抑制。外源性Fgf2+Fgf8+Bmp7在尾纲两栖动物中具有再生诱导作用，可以弥补去神经支配对副胚形成的影响。我们的研究结果表明，蝾螈附属物的再生是由常见的Fgf和bmp信号级联调控的。
Gill regeneration has not been well studied compared to regeneration of other appendages, such as limb and tail regeneration. Here, we focused on axolotl gill regeneration and found that Fgf- and Bmp-signaling are involved in their gill regeneration mechanism. Axolotls have three pairs of gill rami, and each gill ramus has multiple gill filaments. The gills consist of mesenchyme rich in extracellular matrix and epidermis. The gill nerves are supplied from the trigeminal ganglia located in the head. Denervation resulted in no gill regeneration responses. Nerves and gills expressBmpandFgfgenes, and treating animals with Fgf- and Bmp-signaling inhibitors results in phenotypes similar to those seen in denervated gills. Inducing an accessory appendage is a standard assay in amphibian regeneration research. In our study, an accessory gill could be induced by lateral wounding, suggesting that thin axon fibers and mesenchymalFgfs andBmps contributed to the induction of the accessory structure. Such accessory gill induction was inhibited by the denervation. Exogenous Fgf2+Fgf8+Bmp7, which have been determined to function as a regeneration inducer in urodele amphibians, could compensate for the effects denervation has on accessory blastema formation. Our findings suggest that regeneration of appendages in axolotls is regulated by common Fgf- and Bmp-signaling cascades.