Probing skin pigmentation changes with transient absorption imaging of eumelanin and pheomelanin
Probing skin pigmentation changes with transient absorption imaging of eumelanin and pheomelanin
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DOI：
10.1117/1.2976424
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发表时间：
2008-09-01
影响因子：
3.5
通讯作者：
Warren, Warren S.
中科院分区：
文献类型：
--
作者：
Fu, Dan;Ye, Tong;Warren, Warren S.
黑色素是一类广泛存在的具有重要生物学意义的天然色素，在皮肤的光保护中起着重要的作用。黑色素生成的变化可能有助于临床诊断黑色素瘤的进展阶段。以前，我们展示了一种新的方法，成像黑色素分布在组织中的双色瞬态吸收显微镜。在这里，我们将这项研究扩展到更长的波长，并表明我们能够以更高的信噪比（SNR）对固定的薄皮肤切片中的黑色素进行成像，并展示epimode成像。我们发现，光热效应和长寿命的激发态可以有助于长寿命的信号。真黑素和褐黑素表现出明显不同的长寿命激发态吸收。这种差异使我们能够以亚细胞分辨率绘制出它们各自在组织样本中的分布。该技术可为黑素细胞恶性转化的诊断提供有价值的信息。(C)2008年，由光学仪器工程师协会（Society of Photo-Optical Instrumentation Engineers）主办。
As some of the most ubiquitous and biologically important natural pigments, melanins play essential roles in the photoprotection of skin. Changes in melanin production could potentially be useful for clinical diagnosis of the progression stage of melanoma. Previously we demonstrated a new method for imaging melanin distribution in tissue with two-color transient absorption microscopy. Here we extend this study to longer wavelengths and show that we are able to image melanin in fixed thin skin slices with higher signal-to-noise ratios (SNRs) and demonstrate epimode imaging. We show that both photothermal effects and long-lived excited states can contribute to the long-lived signal. Eumelanin and pheomelanin exhibit markedly different long-lived excited state absorption. This difference should enable us to map out their respective distribution in tissue samples with subcellular resolution. This technique could provide valuable information in diagnosing the malignant transformation of melanocytes. (C) 2008 Society of Photo-Optical Instrumentation Engineers.