THE EFFICACY OF DIRECT, 24-HOUR CULTURE, AND MITOTIC SYNCHRONIZATION METHODS FOR CYTOGENETIC ANALYSIS OF BONE-MARROW IN NEOPLASTIC HEMATOLOGIC DISORDERS
THE EFFICACY OF DIRECT, 24-HOUR CULTURE, AND MITOTIC SYNCHRONIZATION METHODS FOR CYTOGENETIC ANALYSIS OF BONE-MARROW IN NEOPLASTIC HEMATOLOGIC DISORDERS
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DOI：
10.1016/0165-4608(85)90032-9
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发表时间：
1985-01-01
影响因子：
--
通讯作者：
PIERRE, RV
中科院分区：
文献类型：
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作者：
DEWALD, GW;BRODERICK, DJ;PIERRE, RV
我们对90例疑似血液学疾病患者的骨髓抽取进行了不同的细胞遗传学方法处理，以确定是否有任何方法更有可能显示染色体异常克隆或产生质量更好的中期。所有标本均采用直接法处理，无丝裂原培养24小时；50份标本也采用紫翅虫有丝分裂同步法处理。在每种情况下，显微镜载玻片由处理技术人员编码，并由另外两名经验丰富的细胞遗传学技术人员分析。直到所有90个标本被分析后，调查人员才知道结果。除一个标本仅在直接制备中发现染色体异常克隆外，三种方法间核型无明显差异。此外，三种方法在中期质量或数量上的差异无统计学意义；然而，24小时无刺激培养比有丝分裂同步过程产生更多的中期。不一致的最大来源是由于一个测试没有产生中期，或者当其他测试产生成功的研究时，结果不确定。我们建议在日常实践中至少应使用两种不同的方法，其中至少一种方法是直接技术可能是最好的。
Bone marrow aspirates from 90 patients suspected of having a hematologic disorder were processed by using different cytogenetic methods to determine if any procedure was more likely to reveal a chromosomally abnormal clone or produce better-quality metaphases. All specimens were processed by a direct technique and 24-h culture without mitogens; 50 specimens were also processed by an amethopterin mitotic synchronization method. In each case, the microscope slides were coded by the processing technologist and analyzed by two other experienced cytogenetic technologists. The results were not known to any of the investigators until all 90 specimens were analyzed. With the exception of one specimen, in which a chromosomally abnormal clone was identified only in the direct preparation, no apparent differences were found in the karyotypes among the three methods. Also, the differences in the quality or number of metaphases found among the three methods were not statistically significant; however, 24-h unstimulated cultures produced more metaphases than the mitotic synchronization procedure. The greatest source of discordance was caused by one test yielding either no metaphases or an uncertain result when the other tests produced a successful study. We suggest that in routine practice at least two different methods should be used, and it may be best if at least one of these methods is a direct technique.