Selective glutathione depletion on function and structure of the isolated perfused rat kidney.
Selective glutathione depletion on function and structure of the isolated perfused rat kidney.
复制标题
选择性谷胱甘肽消耗对离体灌注大鼠肾脏功能和结构的影响。
DOI：
10.1038/ki.1983.142
复制
发表时间：
1983
影响因子：
19.6
通讯作者：
Epstein,FH
中科院分区：
文献类型：
--
作者：
Brezis,M;Rosen,S;Silva,P;Epstein,FH
方法肾灌流。实验动物均为雄性SD大鼠，体重330~500g。这些大鼠被喂以大鼠食物(普利纳)，并允许自由饮水。各组动物均以100 mg/kg的乳杆菌素®腹腔注射麻醉。右肾的灌注按照Ross、Epstein和Leaf的描述进行[3]。导管顶端的灌流压力维持在85毫米汞柱。标准灌流介质为Krebs-Henseleit生理盐水，充气95%02/5%二氧化碳，含5M葡萄糖和6.7g%牛
Methods Kidney perfusion. Male Sprague-Dawley rats, weighing 330 to 500 g, were used for all experiments. The rats were fed rat chow (Purina) and allowed free access to water. The animals were anesthetized intraperitoneally with mactin®, 100 mg/kg. Perfusion of the right kidney was performed as described by Ross, Epstein, and Leaf [3]. Perfusion pressure was maintained at 85 mm Hg at the tip of the cannula. The standard perfusion medium was Krebs-Henseleit physiological saline, gassed with 95% 02/5% CO2 containing 5 m glucose and 6.7 g% bovine