Corticosteroid therapy and airflow obstruction influence the bronchial microbiome, which is distinct from that of bronchoalveolar lavage in asthmatic airways.
Corticosteroid therapy and airflow obstruction influence the bronchial microbiome, which is distinct from that of bronchoalveolar lavage in asthmatic airways.
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DOI：
10.1016/j.jaci.2015.10.017
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发表时间：
2016-05
期刊：
影响因子：
--
通讯作者：
White SR
中科院分区：
文献类型：
--
作者：
Denner DR;Sangwan N;Becker JB;Hogarth DK;Oldham J;Castillo J;Sperling AI;Solway J;Naureckas ET;Gilbert JA;White SR
The lung has a diverse microbiome that is modest in biomass. This microbiome differs in asthmatic patients compared to control subjects, but the effects of clinical characteristics on the microbial community composition and structure are not clear. We examined whether the composition and structure of the lower airway microbiome correlated with clinical characteristics of chronic, persistent asthma including airflow obstruction, use of corticosteroid medications, and presence of airway eosinophilia. DNA was extracted from endobronchial brushings and bronchoalveolar lavage fluid collected from 39 asthmatic and 19 control subjects, along with negative control samples. 16S rRNA V4 amplicon sequencing was employed to compare the relative abundance of bacterial genera to clinical characteristics. Differential feature selection analysis revealed significant differences in microbial diversity between asthmatic and control brush and lavage samples. Lactobacillus, Pseudomonas, and Rickettsia were significantly enriched in asthmatic samples; while Prevotella, Streptococcus, and Vellonella were enriched in the control brushing samples. Generalized linear models (GLM) on brush samples demonstrated oral corticosteroid usage as an important factor affecting the relative abundance of the taxa significantly enriched in asthmatic patients. In addition, bacterial alpha-diversity in brush samples from asthmatic subjects was correlated with FEV1 and with the proportion of lavage eosinophils. The diversity and composition of the bronchial airway microbiome of asthmatic patients is distinct from that of control, non-asthmatic patients and is influenced by worsening airflow obstruction and corticosteroid usage.
