Broadband coherent Raman imaging for multiplexed detection
Broadband coherent Raman imaging for multiplexed detection
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用于多重检测的宽带相干拉曼成像
DOI：
10.1117/12.875180
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发表时间：
2011
期刊：
影响因子：
--
通讯作者：
Littleton B
中科院分区：
文献类型：
--
作者：
Littleton B
荧光发色团的拉曼光谱通常具有许多光谱特征，即使它们的电子光谱相似，这些光谱特征在染料之间也明显不同。这种高信息含量使得可以基于生物标志物的拉曼光谱来区分生物标志物。因此，相干反斯托克斯拉曼散射可以允许同时测量比荧光成像可能的更多的生物标志物，同时避免漂白和样品自发荧光。我们已经建立了一个宽带汽车显微光谱仪，以展示汽车多路复用的原理，并探讨将该系统应用于生物样品研究的潜力。
The Raman spectrum of a fluorescent chromophore typically has many spectral features, which differ markedly between dyes even if their electronic spectra are similar. This high information content makes it possible to distinguish biomarkers based on their Raman spectra. Coherent anti-Stokes Raman scattering may therefore allow for the simultaneous measurement of more biomarkers than is possible with fluorescent imaging, while avoiding bleaching and sample autofluorescence. We have built a broadband CARS microspectrometer to demonstrate the principle of CARS multiplexing and investigate the potential to apply the system to studies of biological samples.