DeaD contributes to Pseudomonas aeruginosa virulence in a mouse acute pneumonia model
DeaD contributes to Pseudomonas aeruginosa virulence in a mouse acute pneumonia model
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DeaD 有助于小鼠急性肺炎模型中的铜绿假单胞菌毒力
DOI：
10.1093/femsle/fnw227
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发表时间：
2016
影响因子：
2.1
通讯作者：
Jin Yongxin
中科院分区：
文献类型：
--
作者：
Tan Hao;Zhang Lu;Zhao Qiang;Chen Ronghao;Liu Chang;Weng Yuding;Peng Qianqian;Bai Fang;Cheng Zhihui;Jin Shouguang;Wu Weihui;Jin Yongxin
DExD/H 盒 RNA 解旋酶在原核生物和真核生物的各种生物过程中发挥着重要作用。通过筛选具有多种DExD/H盒解旋酶基因突变的铜绿假单胞菌，我们发现deaD是小鼠急性肺炎模型中细菌细胞毒性和毒力所必需的。与野生型菌株及其互补菌株相比，thedeaD突变体在感染过程中诱导的促炎细胞因子、中性粒细胞浸润和肺损伤较少。我们进一步发现DeaD的RNA解旋酶活性是III型分泌系统（T3SS）基因表达所必需的。 T3SS的主要激活剂ExsA的过表达恢复了T3SS基因的表达以及thedeaD突变体的毒力，这表明thedeaD突变体的毒力减弱主要是由于T3SS缺陷所致。总的来说，我们的结果揭示了DeaD在P的毒力中的作用。铜绿假单胞菌。
DExD/H box RNA helicases play essential roles in various biological processes in prokaryotes and eukaryotes. By screeningPseudomonas aeruginosastrains with mutations in various DExD/H box helicase genes, we identified thatdeaDwas required for bacterial cytotoxicity and virulence in a mouse acute pneumonia model. Compared to a wild-type strain and its complementation strain, thedeaDmutant induced less production of proinflammatory cytokines, neutrophil infiltration and lung damage during infection. We further found that the RNA helicase activity of DeaD was required for the expression of type III secretion system (T3SS) genes. Overexpression of ExsA, a master activator of the T3SS, restored the expression of T3SS genes as well as the virulence of thedeaDmutant, suggesting that the attenuated virulence of thedeaDmutant was mainly due to the defective T3SS. Overall, our results reveal a role of DeaD in the virulence ofP. aeruginosa.