Inconsistently impairment of immune function and structural integrity of head kidney and spleen by vitamin A deficiency in grass carp (Ctenopharyngodon idella)
Inconsistently impairment of immune function and structural integrity of head kidney and spleen by vitamin A deficiency in grass carp (Ctenopharyngodon idella)
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草鱼（Ctenopharyngodon idella）维生素 A 缺乏对免疫功能以及头肾和脾脏结构完整性的持续损害
DOI：
10.1016/j.fsi.2020.02.019
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发表时间：
2020-04-01
影响因子：
4.7
通讯作者：
Zhou, Xiao-Qiu
中科院分区：
文献类型：
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作者：
Jiang, Wei-Dan;Zhang, Li;Zhou, Xiao-Qiu
为探讨维生素A(VA)对鱼体免疫功能及头肾、头脾结构完整性的影响，将540尾草鱼随机分为6组，分别饲喂不同水平的VA(0、600、1200、1800、2800和3800IU/kg饲料)，共70d。结果表明，饲料中VA缺乏降低了鱼头、肾和脾的抗菌能力，加重了炎症反应，部分与核因子kappaBp65和雷帕霉素(TOR)信号转导途径有关。同时，VA缺乏导致细胞氧化损伤、细胞凋亡和紧密连接(TJs)中断，这部分归因于NF-E2相关因子2(Nrf2)信号通路下调介导的抗氧化能力、p38丝裂原活化蛋白激酶(P38MAPK)信号上调介导的细胞凋亡和肌球蛋白轻链激酶(MLCK)信号介导的紧密连接破坏(TJ)。综上所述，目前的研究首次证明，VA缺乏会降低鱼类的免疫功能，损害头、肾和脾的结构完整性。
To investigate effects of vitamin A (VA) on fish immune function and structural integrity in the head kidney and spleen of fish, total of 540 on-growing grass carp (Ctenopharyngodon idella) were divided into six groups, feeding graded levels of VA (0, 600, 1200, 1800, 2800 and 3800 IU/kg diet) for 70 days. Results showed that dietary VA deficiency depressed antibacterial ability and aggravated inflammatory response partially linked to nuclear factor kappa B p65 (NF-kappa B p65) and target of rapamycin (TOR) signaling pathways in the head kidney and spleen of fish. Meanwhile, VA deficiency caused oxidative damage, apoptosis and disruption of tight junctions (TJs), which were partially attributed to the down-regulation of NF-E2-related factor 2 (Nrf2) signaling mediated antioxidant ability, the up-regulation of p38 mitogen-activated protein kinase (p38MAPK) signaling mediated apoptosis and myosin light chain kinase (MLCK) signaling mediated disruption of tight junctions (TJs). Taken together, current study firstly demonstrated that VA deficiency decreased the immune function and damaged the structural integrity of the head kidney and spleen in fish.