Expression of OPN3 in lung adenocarcinoma promotes epithelial-mesenchymal transition and tumor metastasis
Expression of OPN3 in lung adenocarcinoma promotes epithelial-mesenchymal transition and tumor metastasis
复制标题
OPN3在肺腺癌中的表达促进上皮间质转化和肿瘤转移
DOI：
10.1111/1759-7714.13254
复制
发表时间：
2019-12-05
期刊：
影响因子：
2.9
通讯作者：
Jiang, Zhansheng
中科院分区：
文献类型：
--
作者：
Xu, Chao;Wang, Ruixia;Jiang, Zhansheng
Background: Lung adenocarcinoma is the most common pathological lung cancer and an important cause of cancer-related death. Metastasis is a major underlying reason for poor prognosis of lung adenocarcinoma. Opsin3 (OPN3), a member of the guanine nucleotide-binding protein-coupled receptor superfamily, has been identified to affect the apoptosis of hepatoma cells by modulating the phosphorylation of Akt and Bcl2/Bax. However, the expression and role of OPN3 in lung adenocarcinoma remains unclear.Methods: Opsin3 expression in lung adenocarcinoma tissues was detected by western blot, qPCR, and immunohistochemistry. Changes in cell migration and invasion ability resulting from the change of OPN3 expression level were detected by wound healing and transwell migration assays. Changes in the markers of epithelial-mesenchymal transformation were detected by western blot and qPCR.Results: Opsin3 expression in lung adenocarcinoma tissues was higher than that in normal lung tissues. Patients with high expression of OPN3 had lower survival rates. Owing to overexpression of OPN3, the HCC827 cells showed enhanced invasion and migration ability in vitro. Upon decreasing the expression of OPN3, the invasion and migration ability of the A549 cells decreased.Conclusion: Our study demonstrated for the first time that OPN3 gene enhanced the metastasis in lung adenocarcinoma, and its overexpression promoted epithelial-mesenchymal transition.
