Isolation and characterization of high-buoyant-density proteoglycans from semilunar menisci.
Isolation and characterization of high-buoyant-density proteoglycans from semilunar menisci.
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半月半月板高浮力密度蛋白多糖的分离和表征。
DOI：
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发表时间：
1986
期刊：
影响因子：
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通讯作者：
H. Muir
中科院分区：
文献类型：
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作者：
M. E. Adams;C. Mcdevitt;A. Ho;H. Muir
从成年犬盲肠中提取蛋白多糖，并对其进行纯化，对主要种类进行生化鉴定。大多数蛋白多糖在高浮力密度馏分中分离。经琼脂糖-丙烯酰胺复合凝胶电泳分析，该组分中含有两种蛋白多糖。虽然它们比猪喉透明软骨小，硫酸软骨素和硫酸角质素链较短，碳水化合物与蛋白质的比例较低，但它们与透明质酸的特异性相互作用在功能上与透明软骨相似。
Proteoglycans were extracted from adult canine menisci in high yield and were purified, and the major species were characterized biochemically. Most proteoglycans in menisci were isolated in the high-buoyant-density fraction. By agarose-acrylamide composite gel electrophoresis, two proteoglycans were seen in this fraction. Although they were smaller than those from porcine laryngeal hyaline cartilage and had shorter chondroitin sulphate and keratan sulphate chains and a lower carbohydrate-to-protein ratio, they were functionally similar to those in hyaline cartilage in their specific interaction with hyaluronate.