Biodegradation of di-n-butyl phthalate by a stable bacterial consortium, HD-1, enriched from activated sludge
Biodegradation of di-n-butyl phthalate by a stable bacterial consortium, HD-1, enriched from activated sludge
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由活性污泥中富集的稳定细菌群 HD-1 对邻苯二甲酸二正丁酯进行生物降解
DOI：
10.1016/j.biortech.2012.10.107
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发表时间：
2013-01-01
影响因子：
11.4
通讯作者：
Lu, Zhenmei
中科院分区：
文献类型：
--
作者：
He, Zhixing;Xiao, Hailong;Lu, Zhenmei
从活性污泥中筛选出一株稳定的能矿化邻苯二甲酸二丁酯(DBP)的微生物菌群HD-1。优势菌群为戈登氏菌、伯克霍尔德氏菌。和Achromobacter sp.，通过变性梯度凝胶电泳(DGGE)对其进行鉴定。培养48h后，该联合体对1200 mg/L DBP的矿化率可达90%左右。DBP的最佳降解条件为25~30℃，pH为8.0~9.0。添加酵母(0.5g/L)、乙酸钠(0.5g/L、1.0g/L)、Brij35(0.2%、0.5%、1.0%)或Triton X-100(0.2%)可促进DBP的降解。邻苯二甲酸二甲酯(DMP)和邻苯二甲酸二乙酯(DEP)的存在对DBP的降解速率有影响。在降解过程中，用气相色谱-质谱仪(GC-MS)只能监测到主要中间体邻苯二甲酸。HD-1财团还利用苯酚、邻苯二酚作为唯一的碳和能源。结果表明，该联合体在DBP生物修复中具有良好的应用前景。(C)2012爱思唯尔有限公司。保留所有权利。
HD-1, a stable microbial consortium capable of mineralizing di-n-butyl phthalate (DBP), was developed from activated sludge. The dominant microorganisms in the consortium, Gordonia sp., Burkholderia sp. and Achromobacter sp., were identified by denaturing gradient gel electrophoresis (DGGE). The consortium could mineralize approximately 90% of 1200 mg/L DBP after 48 h of cultivation. The optimal DBP degradation conditions were 25-30 degrees C and pH 8.0-9.0. The addition of yeast (0.5 g/L), sodium acetate (0.5 g/L, 1.0 g/L), Brij 35 (0.2%, 0.5%, 1.0%), or Triton X-100 (0.2%) enhanced DBP degradation. The DBP degradation rate was influenced by the presence of dimethyl phthalate (DMP) and diethyl phthalate (DEP). Only one main intermediate, phthalic acid, could be monitored by gas chromatography-mass spectrometry (GC-MS) during the degradation process. The HD-1 consortium also utilized phenol, o-dihydroxybenzene as the sole carbon and energy source. The results indicate the consortium may represent a promising application for DBP bioremediation. (C) 2012 Elsevier Ltd. All rights reserved.