Photosystem I reaction centers from maize bundle-sheath and mesophyll chloroplasts lack subunit III.
Photosystem I reaction centers from maize bundle-sheath and mesophyll chloroplasts lack subunit III.
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玉米束鞘和叶肉叶绿体的光系统 I 反应中心缺乏亚基 III。
DOI：
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发表时间：
1986
期刊：
影响因子：
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通讯作者：
I. Ohad
中科院分区：
文献类型：
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作者：
R. Nechushtai;G. Schuster;N. Nelson;I. Ohad
从C4玉米叶肉和束鞘叶绿体中分离出光系统I反应中心。两种制剂均不含叶绿素b，光谱性质和叶绿素/P700比值与从C3植物中分离的光系统I反应中心相同。叶肉和束鞘的光系统I反应中心由6个亚基组成，表观分子质量分别为70kda、20kda、17kda、16kda、10kda和8kda，与菠菜的光系统I反应中心I、II、IV、V、VI和VII亚基对应，并与针对菠菜亚基产生的抗体进行免疫交叉反应。在整个类囊体或纯化的束鞘和叶肉叶绿体反应中心均未发现与针对菠菜III亚基的抗体产生交叉反应。综上所述，玉米束鞘和叶肉类囊体的光系统I反应中心是相同的，并且缺乏目前所有C3植物中所存在的III亚基对应的多肽。
Photosystem I reaction centers were isolated from mesophyll and bundle-sheath chloroplasts of the C4 maize plant. Both preparations were found to be free of chlorophyll b and to have the same spectral properties and chlorophyll/P700 ratio as photosystem I reaction centers isolated from C3 plants. Photosystem I reaction centers from both mesophyll and bundle sheath were found to consist of six subunits with apparent molecular masses of about 70 kDa, 20 kDa, 17 kDa, 16 kDa, 10 kDa and 8 kDa, corresponding to photosystem I reaction center subunits I, II, IV, V, VI and VII of spinach, as tested by their immunological cross-reactivity with antibody raised against the respective spinach subunits. No cross-reactivity was found with antibodies raised against subunit III of spinach, either in whole thylakoids or purified reaction centers of both bundle-sheath and mesophyll chloroplasts. It is concluded that photosystem I reaction centers of bundle-sheath and mesophyll thylakoids of maize are identical and lack the polypeptide corresponding to subunit III present in all C3 plants so far tested.