Zearalenone induces apoptosis and inhibits proliferation in porcine ileal Peyer's patch lymphocytes
Zearalenone induces apoptosis and inhibits proliferation in porcine ileal Peyer's patch lymphocytes
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DOI：
10.1515/pjvs-2015-0020
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发表时间：
2015-01-01
影响因子：
0.8
通讯作者：
Poniatowska-Broniek, G.
中科院分区：
文献类型：
--
作者：
Obremski, K.;Poniatowska-Broniek, G.
玉米赤霉烯酮（ZEN）是诱导细胞凋亡的最具活性的天然雌激素化合物之一。这项研究是由食品和饲料中广泛存在的ZEN以及对低剂量ZEN对免疫系统影响的有限了解所推动的。本研究的目的是验证这一假设，即低剂量的ZEN有助于诱导细胞凋亡和抑制增殖的淋巴细胞的生发中心（GC）的回肠派尔集合淋巴结（IPP）在猪。实验在30头雌性波兰大白色猪上进行，2月龄，体重15-18 kg，分为两组：对照组（C，n=15）和实验组（Z，n=15）。在暴露于ZEN的第14、28和42天（100 μ g/kg/天），在HE染色标本中对IPP GC的细胞凋亡进行组织学评价，通过活性半胱天冬酶-3染色进行化学染色，并在基于单克隆抗体和双克隆抗体检测的ELISA中进行细胞凋亡评价。通过HE染色标本中的有丝分裂检测、PCNA染色的免疫组化和检测B细胞对LPS的促有丝分裂反应的MTT四唑盐比色法在组织学上评估增殖。暴露于低剂量的ZEN数周，加强了IPP淋巴细胞的凋亡和减弱了增殖。ZEN通过降低B细胞上CD 21+的表达和增加B-1细胞群的百分比来影响肠道相关淋巴组织（GALT）。
Zearalenone (ZEN) is one of the most active natural estrogenic compounds that induces apoptosis. This study has been prompted by the widespread occurrence of ZEN in food and feed and limited knowledge about the effects of exposure to low doses of ZEN on the immune system. The aim of the study was to verify the hypothesis that low doses of ZEN contribute to induction of apoptosis and inhibition of proliferation in lymphocytes of the germinal centers (GC) of ileal Peyer's patches (IPP) in pigs. The experiment was performed on 30 female Polish Large White pigs, aged 2 months, with body weight of 15-18 kg, divided into two groups: control (C, n=15) and experimental (Z, n=15). On days 14, 28 and 42 of exposure to ZEN (100 mu g kg(-1) feed day(-1)), apoptosis in IPP GC was evaluated histologically in HE-stained specimens, immunohistochemically by active caspase-3 staining and in mononucleosome and oligonucleosome detection-based ELISA. Proliferation was evaluated histologically by mitosis detection in HE-stained specimens, immunohistochemically by PCNA staining and in the MTT tetrazolium salt colorimetric assay detecting mitogenic responses of B cells to LPS. Exposure to low doses of ZEN for several weeks intensified apoptosis and weakened proliferation in IPP lymphocytes. ZEN influences gut-associated lymphoid tissue (GALT) by decreasing the expression of CD21+ on B cells and by increasing the percentage of B-1 cell populations.