A sequence-ready physical map of a region of 12q24.1
A sequence-ready physical map of a region of 12q24.1
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DOI：
10.1006/geno.1997.4888
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发表时间：
1997-10-15
期刊：
影响因子：
4.4
通讯作者：
Kucherlapati, R
中科院分区：
文献类型：
--
作者：
Renault, B;Hovnanian, A;Kucherlapati, R
我们开发了一个人类染色体12q24.1部分的序列图。我们利用一些序列标记位点（STS）标记hom 12q24.1筛选大插入细菌染色体文库和染色体la特异性粘粒文库。通过STS-含量分析将克隆组装成连续组（重叠群）。通过获得细菌克隆的末端序列、产生额外的STS、重新筛选文库并筛选额外克隆中STS的存在来延伸重叠群。所得重叠群覆盖近2 Mb的DNA，并提供16 kb的平均标记分辨率。基于STS内容，我们开发了克隆子集的指纹。STS内容和指纹数据允许我们定义克隆的最小路径。这些克隆被用于对12号染色体的这一部分进行测序。该重叠群包含共济失调蛋白2基因，并且它覆盖了携带负责Darier病的基因的间隔。(C)1997学术出版社。
We developed a sequence-ready map of a part of human chromosome 12q24.1. We utilized a number of sequence-tagged site (STS) markers hom 12q24.1 to screen large insert bacterial chromosome libraries and a chromosome la-specific cosmid library. The clones were assembled into contiguous sets (contigs) by STS-content analysis. Contigs were extended by obtaining end sequences of bacterial clones, generation of additional STSs, rescreening the libraries, and screening the additional clones for the presence of STSs. The resulting contig covers nearly 2 Mb of DNA and provides an average marker resolution of 16 kb. Based on the STS content, we developed fingerprints of a subset of clones. The STS content and fingerprint data allowed us to define a minimal thing path of clones. These clones are being used to sequence this part of chromosome 12. This contig contains the Ataxin 2 gene, and it covers the interval harboring the gene responsible for Darier disease. (C) 1997 Academic Press.