Estimation of postmortem interval using attenuated total reflectance: Fourier transform infrared spectroscopy in adipose tissues
Estimation of postmortem interval using attenuated total reflectance: Fourier transform infrared spectroscopy in adipose tissues
复制标题
使用衰减全反射率估计死后间隔：脂肪组织中的傅里叶变换红外光谱
DOI：
10.4103/jfsm.jfsm_47_18
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发表时间：
2019-01
影响因子：
--
通讯作者：
Shuanliang Fan
中科院分区：
文献类型：
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作者：
Zhenyuan Wang;Haohui Zhang;Qi Wang;Kai Zhang;Ruina Liu;Shuanliang Fan
死亡时间的推断在法医学研究中具有重要意义。虽然许多研究已经检查了各种组织随时间的化学变化，但没有使用脂肪组织中的光谱方法的报告。在这项研究中，衰减全反射-傅里叶变换红外光谱（ATR-FTIR）被用来收集全面的生化信息，从人体脂肪组织在体外不同的时间。此后，将小鼠用作体内实验的样品，用于对PMI进行更详细的研究。然后，偏最小二乘（PLS）模型的PMI估计的基础上获得的光谱数据集的小鼠样品。与C=O产生的脂质和游离脂肪酸的光谱变量是最容易受到PMI。此外，PLS模型似乎实现了令人满意的预测，交叉验证的均方根误差为1.78天，并确定了该模型的可靠性，在外部验证集的预测均方根误差为1.87天。研究表明，ATR-FTIR方法在PMI估计中的应用脂肪组织的可能性。
Estimation of postmortem interval (PMI) is vitally important in forensic investigations. Although many studies have examined the chemical changes of various tissues over time, no reports using spectroscopic methods in adipose tissue are available. In this study, attenuated total reflectance–Fourier transform infrared (ATR–FTIR) spectroscopy was utilized to collect comprehensive biochemical information from human adipose tissues in vitro at different times. Thereafter, mice were used as samples for in vivo experiments for more detailed studies on PMI. Then, partial least squares (PLS) model for PMI estimation was established based on the acquired spectral dataset of mouse samples. The spectral variable associated with C=O arising from lipids and free fatty acids was most susceptible to PMI. Moreover, the PLS model appeared to achieve a satisfactory prediction with a root mean square error of cross-validation of 1.78 days, and the reliability of the model was determined in an external validation set with a root mean square error of prediction of 1.87 days. The study shows the possibility of application of ATR–FTIR methods in PMI estimation using adipose tissue.