Glutamate/aspartate and leu-enkephalin immunoreactivity in mammillothalamic projection neurons of the rat
Glutamate/aspartate and leu-enkephalin immunoreactivity in mammillothalamic projection neurons of the rat
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DOI：
10.1016/s0361-9230(98)00077-x
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发表时间：
1998-12-01
影响因子：
3.8
通讯作者：
Sanz, JM
中科院分区：
文献类型：
--
作者：
Gonzalo-Ruiz, A;Morte, L;Sanz, JM
我们已经使用逆行运输和免疫组织化学研究了谷氨酸、天冬氨酸和脑啡肽样免疫反应途径，从乳头核到丘脑前核。将小麦胚芽凝集素与辣根过氧化物酶结合注射到丘脑前外侧核，导致双侧乳房核的细胞体逆行标记。而丘脑前腹侧核注射导致同侧内侧乳头核的神经元逆行标记。在分别对谷氨酸、天冬氨酸和脑啡肽免疫反应的三个平行系列中，50-60%的逆行标记的细胞体也对谷氨酸、50-60%的天冬氨酸和40-50%的脑啡肽进行了免疫标记。脑啡肽免疫反应神经元可能与谷氨酸/天冬氨酸神经元重合或构成单独的群体。这些结果与乳丘投射神经元可能使用谷氨酸和/或天冬氨酸和脑啡肽作为神经递质的可能性相一致。(C) 1999 Elsevier Science Inc.；
We have used retrograde transport and immunohistochemistry to study glutamate, aspartate, and enkephalin-like immunoreactive pathways from the mammillary nuclei to the anterior nuclei of the thalamus, Injections of wheat germ agglutinin conjugated to horseradish peroxidase into the anterodorsal thalamic nucleus resulted in retrogradely labelled cell bodies in the lateral mammillary nucleus, bilaterally, whereas injections into the anteroventral thalamic nucleus resulted in retrogradely labelled neurons in the ipsilateral medial mammillary nucleus. In three parallel series of sections immunoreacted for glutamate, aspartate, and enkephalin, respectively, 50-60% of the retrogradely labelled cell bodies were also immunolabelled for glutamate, 50-60% for aspartate, and 40-50% for enkephalin, The enkephalin-immunoreactive neurons may coincide with or constitute a separate population from the glutamate/aspartate-containing neurons. These results are compatible with the possibility that mammillothalamic projection neurons may use glutamate and/or aspartate and enkephalin as neurotransmitters. (C) 1999 Elsevier Science Inc.