LEUKOCYTE MARGINATION AND DEFORMATION IN MESENTERIC VENULES OF RAT
LEUKOCYTE MARGINATION AND DEFORMATION IN MESENTERIC VENULES OF RAT
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DOI：
10.1152/ajpheart.1989.256.6.h1667
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发表时间：
1989-06-01
影响因子：
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通讯作者：
LIPOWSKY, HH
中科院分区：
文献类型：
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作者：
FIRRELL, JC;LIPOWSKY, HH
在大鼠肠系膜的小静脉（直径20-60微米）中研究了白色血细胞（WBC）的边集和滚动，以检查WBC滚动速度（Vwbc）、流量（Fwbc，细胞/分钟）和变形之间的关系;以及红细胞（RBC）速度（Vrbc）、壁剪切率（γ）和微血管血细胞比容（Hmicro）之间的关系。对于1.0小于或等于Vrbc小于或等于5.0 mm/s的范围，Vwbc保持恒定（等于50微米/s）。当γ范围为50 - 800 s-1时，沿小静脉壁沿着滚动的WBC的体积通量（标准化为全身WBC浓度）从总管腔通量的30%非线性降低至5%，并且与Fwbc vs. γ相比，与γ的相关性更强。如轧制体积通量所示，除了Hmicro大于50%时边缘化增加之外，边缘化受Hmicro的影响很弱。在WBC与内皮（EC）滚动接触期间，根据其长度和高度定量WBC的变形。随着γ从50增加到800 s-1，WBC伸长到其估计未变形直径的140%，WBC和EC之间的接触面积增加了3.6倍。这些数据表明，随着WBC沿着的γ滚动增加，EC降低，因为循环群体中仅最易变形的WBC持续保持与内皮的接触。可以得出结论，Vwbc是不变的伽玛海拔，因为更大的变形，滚动白细胞在高剪切力的结果在更大的粘附力伴随着白细胞-EC接触面积的增加，衰减增加Vwbc。
White blood cell (WBC) margination and rolling were studied in venules (20-60 microns in diameter) in rat mesentery to examine the relationship between WBC rolling velocity (Vwbc), flux (Fwbc, cells/min), and deformation; and red blood cell (RBC) velocity (Vrbc), wall shear rate (gamma), and microvessel hematocrit (Hmicro). For a range of 1.0 less than or equal to Vrbc less than or equal to 5.0 mm/s, Vwbc remained constant (congruent to 50 microns/s). The volume flux of WBCs rolling along the venular wall (normalized to systemic WBC concentration) decreased nonlinearly from 30 to 5% of total luminal flux as gamma ranged from 50 to 800 s-1 and was more strongly correlated with gamma compared with Fwbc vs. gamma. As indicated by the rolling volumetric flux, margination was weakly affected by Hmicro, except for Hmicro greater than 50% where margination increased. Deformation of WBCs was quantitated during their rolling contact with the endothelium (EC) in terms of their length and height. As gamma increased from 50 to 800 s-1, WBCs elongated to 140% of their estimated undeformed diameter, and the contact area between WBC and EC increased 3.6-fold. These data suggest that with increased gamma rolling of WBCs along the EC decreases, since only the most deformable WBCs of the circulating population persist in maintaining contact with the endothelium. It is concluded that Vwbc is invariant with elevations in gamma, since the greater deformations of rolling WBCs at high shear result in greater adhesive forces attendant to increased WBC-EC contact area, which attenuate increases in Vwbc.