Genetic complementation in Cryptococcus neoformans
Genetic complementation in Cryptococcus neoformans
复制标题
新型隐球菌的基因互补
DOI：
10.1128/jb.166.3.924-929.1986
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发表时间：
1986
影响因子：
3.2
通讯作者：
K. Kwon
中科院分区：
文献类型：
--
作者：
W. Whelan;K. Kwon
设计了一种用于真菌新型隐球菌的互补试验。互补是由a B X Ab型杂交产生的原养异核体的生长发出信号的，其中a和B代表遗传标记ilv 1、cys 1、cys 2和cys 3中的任意两个。克隆的互补异核体形成了含有菌丝和酵母细胞的特征菌丝菌落。异核体来源的酵母有三种：亲本单倍体、重组单倍体和二倍体。
A complementation test was devised for the fungus Cryptococcus neoformans. Complementation was signalled by the growth of prototrophic heterokaryons generated in crosses of the type aB X Ab, where a and b represent any two of the genetic markers ilv1, cys1, cys2, and cys3. The cloned complementing heterokaryons formed characteristic hyphal colonies that contained both hyphae and yeast cells. The heterokaryon-derived yeasts were of three kinds: parental haploids, recombinant haploids, and diploids.