Preparation of mouse pituitary immunogen for the induction of experimental autoimmune hypophysitis.
Preparation of mouse pituitary immunogen for the induction of experimental autoimmune hypophysitis.
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DOI：
10.3791/2181
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发表时间：
2010-12
期刊：
影响因子：
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通讯作者：
Shey-Cherng Tzou;Melissa A. Landek-Salgado;Hiroaki Kimura;P. Caturegli
中科院分区：
文献类型：
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作者：
Shey-Cherng Tzou;Melissa A. Landek-Salgado;Hiroaki Kimura;P. Caturegli
自身免疫性垂体炎是由自身免疫引起或伴随的垂体慢性炎症（1）。它传统上被认为是一种罕见的疾病，但近年来报告显着增加。事实上，垂体炎在使用阻断T淋巴细胞上表达的抑制性受体（如CTLA-4（2）和PD-1受体）的抗体治疗的癌症患者中并不罕见。通过向小鼠注射垂体蛋白与佐剂的混合物，可以在实验中诱导自身免疫性垂体炎（3）。在这篇视频文章中，我们演示了如何从小鼠脑垂体中提取蛋白质，以及如何将其制备成适合诱导SJL小鼠自身免疫性垂体炎的形式。
Autoimmune hypophysitis is a chronic inflammation of the pituitary gland caused or accompanied by autoimmunity(1). It has traditionally been considered a rare disease but reporting has increased markedly in recent years. Hypophysitis, in fact, develops not uncommonly as a "side effect" in cancer patients treated with antibodies that block inhibitory receptors expressed on T lymphocytes, such as CTLA-4(2) and PD-1 receptors. Autoimmune hypophysitis can be induced experimentally by injecting mice with pituitary proteins mixed with an adjuvant(3). In this video article we demonstrate how to extract proteins from mouse pituitary glands and how to prepare them in a form suitable for inducing autoimmune hypophysitis in SJL mice.