Real-time detection of hyperosmotic stress response in optically trapped single yeast cells using Raman microspectroscopy.
Real-time detection of hyperosmotic stress response in optically trapped single yeast cells using Raman microspectroscopy.
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DOI：
10.1021/ac048359j
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发表时间：
2005-03
影响因子：
7.4
通讯作者：
G. Singh;C. Creely;G. Volpe;H. Grötsch;D. Petrov
中科院分区：
文献类型：
--
作者：
G. Singh;C. Creely;G. Volpe;H. Grötsch;D. Petrov
Living cells survive environmentally stressful conditions by initiating a stress response. We monitored changes in the Raman spectra of optically trapped Saccharomyces cerevisiae yeast cell under normal, heat-treated, and hyperosmotic stress conditions. It is shown that when glucose was used to exert hyperosmotic stress, two chemical substances-glycerol and ethanol-can be monitored in real time in a single cell.
