Identification and mapping of open chromatin regions within a 140 kb polygenic locus of human chromosome 19 using E-coli Dam methylase
Identification and mapping of open chromatin regions within a 140 kb polygenic locus of human chromosome 19 using E-coli Dam methylase
复制标题
DOI：
10.1007/s10709-006-0026-1
复制
发表时间：
2007-05-01
期刊：
影响因子：
1.5
通讯作者：
Sverdlov, Eugene
中科院分区：
文献类型：
--
作者：
Bulanenkova, Svetlana;Snezhkov, Eugene;Sverdlov, Eugene
利用大肠杆菌Dam甲基化酶基因的瞬时表达和甲基化GATC位点的分布分析，我们研究了HEK-293细胞中140 kb长的人类基因组片段中开放染色质区域的分布。甲基化位点主要分布在基因的内含子、外显子和基因间区，且沿DNA的分布不均匀。Dam甲基化GATC位点存在高密度和低密度区域，可能分别对应于“开放”和“封闭”染色质区域，并与所研究的基因组位点的功能谱相对应。Dam甲基化谱也与该位点基因的转录活性大致一致。此外，Dam甲基化酶可接近的DNA区域与DNA酶I敏感的DNA区域明显重合。
Using transient expression of the E. coli Dam methylase gene and analysis of the distribution of methylated GATC sites, we studied the distribution of open chromatin regions within a 140 kb long human genome segment in HEK-293 cells. Dam methylated sites were found in gene introns, exons, and intergenic regions, and their distribution along DNA was uneven. There were regions of high and low density of Dam methylated GATC sites, presumably corresponding to "open'' and "closed'' chromatin regions, respectively, and to the functional profile of the genomic locus under study. The Dam methylation profile was also generally in agreement with transcriptional activity of genes in the locus. Moreover, DNA regions accessible to Dam methylase apparently coincided with those hypersensitive to DNase I.