The amino acid sequence of ragweed pollen allergen Ra5.
The amino acid sequence of ragweed pollen allergen Ra5.
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豚草花粉过敏原 Ra5 的氨基酸序列。
作者：
L. Mole;L. Goodfriend;C. B. Lapkoff;J. Kehoe;J. Capra
豚草花粉过敏原 Ra5 的完整氨基酸序列已确定。过敏原 Ra5 是一种由 45 个残基组成的低分子量蛋白质，源自 Ambrosia elatior（短豚草）。它不含可检测到的碳水化合物或脂质，并具有四个二硫桥。总结构是在 1.4 μmol 材料上测定的，表明当采用自动和手动测序技术以及高灵敏度检测系统的组合时，对相对少量的高纯度材料进行结构分析越来越有可能。这代表了豚草过敏原的第一个完整氨基酸序列，它应该为速发型超敏反应领域的许多结构功能相关实验提供基础。
The complete amino acid sequence of Ra5, a ragweed pollen allergen, has been determined. Allergen Ra5 is a low molecular weight protein of 45 residues derived from Ambrosia elatior, the short ragweed. It contains no detectable carbohydrate or lipid and has four disulfide bridges. The total structure was determined on 1.4 mumol of material and indicates that structural analysis is increasingly possible on relatively small amounts of highly purified material when a combination of automated and manual sequencing techniques and highly sensitive detection systems is employed. This represents the first complete amino acid sequence of a ragweed allergen and it should provide a basis for many structure-function correlative experiments in the field of immediate hypersensitivity.