Characterization of a Streptococcus species isolated from Siganus guttatus in South China
Characterization of a Streptococcus species isolated from Siganus guttatus in South China
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从华南地区的 Siganus guttatus 中分离出的链球菌的特征
DOI：
10.1016/j.aquaculture.2021.737163
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发表时间：
2021-12
期刊：
影响因子：
4.5
通讯作者：
Lei Shi
中科院分区：
文献类型：
--
作者：
Lili Li;Chong Wang;Rikke Heidemann Olsen;Xianfeng Li;Hecheng Meng;Liwen Xu;Lei Shi
链球菌已成为世界范围内鱼类养殖的重要病原体。本研究对中国南方一种培养的、致病的点滴状石蟹链球菌进行了鉴定。表型、化学分类、16 S rRNA基因测序、系统发育和基因组比较分析表明，该菌株与已鉴定的链球菌属（Streptococcussp.）X13 SY 08来自Murray cod（Maccullochella peelii peelii）。利用全基因组序列预测了该菌的毒力因子，并通过攻毒试验分析了该菌的致病性。在基因组上共鉴定出30个毒力因子。S. X16 XC 17菌株经胸内滴注，在108 CFU/尾时，累积死亡率为10 0%，在104 CFU/尾至107 CFU/尾时，累积死亡率为45%~ 85%。病理组织学检查结果显示，脾脏、肾脏、肝脏和心脏均有病理改变。此外，还开发了一种特异性PCR检测方法来检测该种属。抗生素耐药结果表明，该菌仅对甲氧苄啶-磺胺甲恶唑耐药.本文报道了一种新的链球菌属细菌，它可能是该病的病原体. guttatus，并提供了在随后的研究与这种病原体的毒力和发病机制的见解。
Streptococcus has emerged as an important pathogen in fish farming worldwide. In this study, we characterized aStreptococcusspecies from a cultured, diseasedSiganusguttatusin South China. Phenotypic, chemotaxonomic, 16S rRNA gene sequencing, phylogenetic and genome comparison analysis indicated that this isolate may be considered as same species with previously reportedStreptococcussp. X13SY08 from Murray cod (Maccullochella peelii peelii). We further predicted the virulence factor using whole genome sequence and analyzed the pathogenesis of this species by challenge tests. A total of 30 virulence factors were identified on the genome. Challenge tests ofS. guttatusvia intrathoracic injection of strain X16XC17 exhibited 100% cumulative mortality rates at 108colony forming units (CFU) per fish, and 45% to 85% at 104to 107CFU per fish. The histopathological results showed that the spleen, kidney, liver and heart all had pathological manifestations. Additionally, a specific PCR assay was developed to detect this species. The antibiotic resistance results showed that this species was only resistant to trimethoprim- sulfamethoxazole. This study describes a new species of genusStreptococcusas potential causative agent of diseasedS. guttatus, and offers insights in the subsequent study of the virulence and pathogenesis associated with this pathogen.
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DOI：
--
发表时间：
1990
期刊：
--
影响因子：
--
作者：
D. Woodland
通讯作者：
D. Woodland
DOI：
10.1128/genomea.01470-15
发表时间：
2016-01-07
期刊：
Genome announcements
影响因子：
--
作者：
Liu Y;Zeng R;Weng B;Luo T;Luo Q;Xu L
通讯作者：
Xu L
DOI：
10.1097/pas.0b013e3181805089
发表时间：
2009-02
期刊：
The American Journal of Surgical Pathology
影响因子：
--
作者：
Gwendolyn R. Goss
通讯作者：
Gwendolyn R. Goss
影响因子：
7
作者：
Li, Ruiqiang;Zhu, Hongmei;Wang, Jun
通讯作者：
Wang, Jun
影响因子：
9.4
作者：
David Sue;A. Hoffmaster;T. Popović;P. Wilkins
通讯作者：
David Sue;A. Hoffmaster;T. Popović;P. Wilkins