Nitric oxide mediates Fos expression in the spinal cord induced by mechanical noxious stimulation.
Nitric oxide mediates Fos expression in the spinal cord induced by mechanical noxious stimulation.
复制标题
一氧化氮介导机械伤害性刺激诱导的脊髓中 Fos 的表达。
DOI：
10.1097/00001756-199210000-00005
复制
发表时间：
1992
期刊：
影响因子：
1.7
通讯作者：
Beitz,AJ
中科院分区：
文献类型：
--
作者：
Lee,JH;Wilcox,GL;Beitz,AJ
应用免疫细胞化学方法对Fos蛋白进行定位，分析一氧化氮(NO)在机械伤害性刺激(NS)诱导的脊髓Fos表达中的作用。鞘内注射一氧化氮合酶抑制剂N[omega]-硝基-L-精氨酸甲酯(L-NAME)30min后，对左侧后爪注射机械生理盐水，并与单纯机械生理盐水组大鼠脊髓背角Fos表达进行比较。预先给予L-NAME而不是其立体异构体N[omega]-硝基-D-精氨酸甲酯(D-NAME)，可剂量依赖性地抑制机械伤害性刺激诱导的Fos表达。这些结果表明，NO调节机械伤害性刺激诱导的背角Fos的表达，进一步支持了NO参与脊髓对外周伤害性刺激的伤害性反应的假说。
IMMUNOCYTOCHEMICAL localization of Fos protein was used to analyze the involvement of nitric oxide (NO) in the expression of Fos in the spinal cord, induced by mechanical noxious stimulation (NS). Mechanical NS was applied to the left hindpaw 30 minutes after intrathecal administration of the NO synthase inhibitor, N [omega]-nitro-L-arginine methyl ester (L-NAME) and the resulting Fos expression in the spinal cord dorsal horn was compared with that obtained in rats exposed only to the mechanical NS. Pretreatment with L-NAME but not its stereoisomer N [omega]-nitro-D-arginine methyl ester (D-NAME), produced a dose-dependent suppression of Fos expression induced by mechanical noxious stimulation. These results indicate that NO modulates the expression of Fos in the dorsal horn induced by mechanical noxious stimulation and further support the hypothesis that NO is involved in nociceptive events occurring in the spinal cord in response to a peripheral noxious stimulus.