Responses to ultraviolet-B radiation (280-315 nm) of pea (Pisum sativum) lines differing in leaf surface wax
Responses to ultraviolet-B radiation (280-315 nm) of pea (Pisum sativum) lines differing in leaf surface wax
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DOI：
10.1111/j.1399-3054.1996.tb06695.x
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发表时间：
1996-12-01
影响因子：
6.4
通讯作者：
Wellburn, AR
中科院分区：
文献类型：
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作者：
Gonzalez, R;Paul, ND;Wellburn, AR
为了验证叶表面蜡质影响植物对UV-B反应的假说，选择6个含蜡量不同的栽培豌豆品系，在0和6.5kJ·m(-2)d(-1)植株加权UV-B的背景下进行生长。(-2)S(-1)叶表面蜡质含量最少的4个品系的光合作用有效辐射。但UV-B减少了Scout的表面蜡，那里有最大的蜡沉积。在近轴叶表面，UV-B辐射引起蜡类成分由醇类向酯类和碳氢化合物转移，短链烷基酯同系物与长链烷基酯同系物的比例增加。UV-B没有引起碳氢化合物、伯醇或脂肪酸碳链长度的缩短，蜡量与叶片的UV反射率之间也没有显著的相关性。UV-B诱导了大部分品系展开的叶片和花蕾中的UV吸收化合物的显著增加。UV-B对所有品系的生长均有抑制作用。叶面积(叶加托叶)下降5-30%，植株干重下降12-30%，株高下降24%-38%。在没有任何叶绿素荧光或光合作用速率变化的情况下，生长减慢。UV-B对碳分配格局也没有显著影响。UV-B对生长的影响似乎是由于组织伸展和扩张的变化。事实上，在这项豌豆研究中观察到的许多对UV-B的反应似乎更符合在发育中的组织中表达的间接效应，而不是通过UV-B对成熟组织的直接作用。没有证据表明蜡量或生化与豌豆品系对UV-B辐射的敏感性有关。此外，色素的诱导与生长的变化无关。然而，在未展开的芽组织中含有最多组成色素的品系对升高的UV-B的耐受性最强。
To test the hypothesis that leaf surface wax influences plant responses to UV-B, 6 lines of cultivated pea (Pisum sativum L.), selected as having more or less wax, were grown at 0 or 6.5 kJ m(-2) day(-1) plant weighted UV-B against a background of 850-950 mu mol m(-2) s(-1) photosynthetically active radiation In the 4 lines with least leaf surface wax the amount of wax on adaxial and abaxial leaf surfaces was increased following exposure to 6.5 kJ m(-2) day(-1) UV-B, but UV-B decreased surface wax in Scout, which had the greatest wax deposits. On the adaxial leaf surface, UV-B radiation caused a shift in wax composition from alcohols to esters and hydrocarbons and the ratio of short to long chain length alkyl ester homologues was increased. There was no evidence of a shortening in carbon chain length of hydrocarbons, primary alcohols or fatty acids due to UV-B and no significant correlation between wax amount and UV reflectance from leaves.UV-B induced significant increases in UV-absorbing compounds in the expanded leaves and buds of most lines. UV-B reduced the growth of all lines. Foliage area (leaves plus stipules) declined by 5-30%, plant dry weight by 12-30%, and plant height by 24-38%. Reductions in growth occurred in the absence of any changes in chlorophyll fluorescence or photosynthetic rate. UV-B also had no major effect on carbon allocation patterns. The effects of UV-B on growth appeared to be due to changes in tissue extension and expansion. Indeed, many of the responses to UV-B observed in this study of pea appear more consistent with indirect effects being expressed in developing tissues rather than through the direct action of UV-B on mature tissues.There was no evidence that wax amount or biochemistry was associated with the sensitivity of the lines to UV-B radiation. Furthermore, induction of pigments was not correlated with changes in growth. However, lines with the greatest constitutive amounts of pigments in unexpanded bud tissues were most tolerant of elevated UV-B.