CORRELATION OF INTRATHYMIC TOLERANCE WITH INTRATHYMIC CHIMERISM IN NEONATALLY TOLERIZED MICE
CORRELATION OF INTRATHYMIC TOLERANCE WITH INTRATHYMIC CHIMERISM IN NEONATALLY TOLERIZED MICE
复制标题
新生耐受小鼠胸腺内耐受与胸腺内嵌合的相关性
DOI：
10.1097/00007890-198507000-00014
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发表时间：
1985
期刊：
影响因子：
6.2
通讯作者：
A. Singer
中科院分区：
文献类型：
--
作者：
P. Morrissey;S. Sharrow;Y. Kohno;J. Berzofsky;A. Singer
通过注射半同种异体成体脾细胞可以在新生小鼠中诱导T细胞对同种抗原的耐受。经腹腔注射的小鼠的胸腺迅速变得对注射细胞表达的同种异体抗原无特异性反应，尽管据报道，与外周相比，这种小鼠胸腺中的嵌合水平是微不足道的。本文研究了24只腹腔注射小鼠胸腺的免疫反应性和嵌合程度。在13只老年小鼠中，来自个体小鼠的胸腺细胞对注射的同种异体抗原的增殖反应从强反应性到完全耐受性不等。有趣的是，通过流式显微荧光测定法分析相同的胸腺细胞是否存在接种的同种异体抗原，发现了负相关。也就是说，嵌合程度较高的胸腺是耐受的，而那些细胞较少或无法检测到的胸腺是部分或完全反应的。11只年轻的腹腔注射小鼠的胸腺总是耐受F1接种物的MHC和Mis同种异体抗原。这些小鼠的胸腺细胞的流动显微荧光分析显示，大量的F细胞驻留在小鼠胸腺注射半同种异体细胞的新生儿。这些细胞代表了胸腺细胞在胸腺内分化过程中特异性耐受原的可能来源。
T cell tolerance to alloantigens can be induced in neonatal mice by the injection of semiallogeneic adult spleen cells. The thymus of neonatally injected mice rapidly becomes specifically unresponsive to the alloantigens expressed by the injected cells, even though the level of chimerism in the thymus of such mice is reportedly marginal compared with that of the periphery. In this report, we have studied the immune reactivity and degree of chimerism in the thymuses of 24 neonatally injected mice. In 13 older mice, the proliferative response of the thymocytes from individual mice to the injected alloantigen varied from strong reactivity to complete tolerance. Interestingly, analysis by flow microfluorometry of the same thymocytes for the presence of the inoculated alloantigen revealed an inverse correlation. That is, the thymuses which had a greater degree of chimerism were tolerant and those with fewer or no detectable cells were partially or totally reactive. The thymuses of 11 younger neonatally injected mice were invariably tolerant to both the MHC and Mis alloantigens of the F1 inocula. Analysis by flow microfluorometry of the thymocytes of these mice revealed that significant numbers of F, cells reside in the thymuses of mice injected as neonates with semiallogeneic cells. These cells represent a possible source of specific tolerogen for thymocytes during their intrathymic differentiation.