Triggered amplification by hybridization chain reaction
Triggered amplification by hybridization chain reaction
复制标题
DOI：
10.1073/pnas.0407024101
复制
发表时间：
2004-10-26
影响因子：
11.1
通讯作者：
Pierce, NA
中科院分区：
文献类型：
--
作者：
Dirks, RM;Pierce, NA
我们介绍了杂交链反应（HCR）的概念，其中稳定的DNA单体组装后，暴露于一个目标DNA片段。在这个过程的最简单的版本中，两种稳定的DNA发夹物种在溶液中共存，直到引入引发剂链触发级联的杂交事件，产生类似于交替共聚物的切口双螺旋。HCR产物的平均分子量与引发剂浓度成反比。可以通过将HCR偶联至适体触发物来实现更多样化的识别事件的扩增。该功能允许DNA充当用于生物传感应用的放大换能器。
We introduce the concept of hybridization chain reaction (HCR), in which stable DNA monomers assemble only upon exposure to a target DNA fragment. In the simplest version of this process, two stable species of DNA hairpins coexist in solution until the introduction of initiator strands triggers a cascade of hybridization events that yields nicked double helices analogous to alternating copolymers. The average molecular weight of the HCR products varies inversely with initiator concentration. Amplification of more diverse recognition events can be achieved by coupling HCR to aptamer triggers. This functionality allows DNA to act as an amplifying transducer for biosensing applications.