Utilisation of galacto-oligosaccharides as selective substrates for growth by lactic acid bacteria including Bifidobacterium lactis DR10 and Lactobacillus rhamnosus DR20
Utilisation of galacto-oligosaccharides as selective substrates for growth by lactic acid bacteria including Bifidobacterium lactis DR10 and Lactobacillus rhamnosus DR20
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DOI：
10.1016/s0958-6946(01)00026-7
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发表时间：
2001-01-01
影响因子：
3.1
通讯作者：
Smart, JB
中科院分区：
文献类型：
--
作者：
Gopal, PK;Sullivan, PA;Smart, JB
对两种益生菌菌株乳酸双歧杆菌DR10和鼠李糖乳杆菌DR20进行了利用和生长商业水解乳糖奶粉中存在的半乳糖低聚糖的能力测试。结果清楚地表明，B. lactis DR10优先利用三糖和四糖，而Lb. rhamnosus DR20偏爱聚合度较低的糖，即双糖和单糖。由于半乳糖低聚糖是不可消化的低聚糖，因此体外实验数据表明，如果奶粉中含有的半乳糖低聚糖与奶粉一起食用，可能会促进体内DR10和DR20的生长。对54株乳酸菌，包括双歧杆菌属和乳杆菌属的成员，研究了它们利用乳糖衍生低聚糖的能力。观察到菌株利用低聚糖的能力与乳糖水解酶-半乳糖苷酶的存在之间存在完美的相关性。基于这些观察结果，双歧杆菌属利用半乳糖寡糖的机制被提出，这可能有助于解释这些生物在人类胃肠道生态系统中胜过其他细菌的能力。2001爱思唯尔科学有限公司版权所有。
Two probiotic strains of bacteria Bifidobacterium lactis DR10 and Lactobacillus rhamnosus DR20 were tested for their ability to utilise and grow on galacto-oligosaccharides present in a commercial hydrolysed lactose milk powder. The results clearly demonstrated that B. lactis DR10 preferentially utilises tri- and tetra-saccharides whereas Lb. rhamnosus DR20 prefers sugars with a lower degree of polymerisation, i.e., disaccharides and monosaccharides. Since galacto-oligosaccharides are non-digestible oligosaccharides, this in vitro data suggest that galacto-oligosaccharides present in milk powders are likely to promote growth of DR10 and DR20 in vivo if these strains are consumed in combination with the milk powder. Fifty four strains of lactic acid bacteria, including members from the genera Bifidobacterium and Lactobacillus, were studied for their ability to utilise lactose derived oligosaccharides. A perfect correlation was observed between the ability of a strain to utilise oligosaccharide and the presence of the lactose hydrolysing enzyme beta -galactosidase. Based on these observations, a mechanism for the utilisation of galacto-oligosaccharides in genus bifidobacteria is proposed that may help to explain the ability of these organisms to out-compete other bacteria in the ecosystcm of the human gastro-intestinal tract. (C) 2001 Elsevier Science Ltd. All rights reserved.