Tubular crystals of a photosystem II core complex.
Tubular crystals of a photosystem II core complex.
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光系统 II 核心复合体的管状晶体。
DOI：
10.1006/jmbi.1996.0316
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发表时间：
1996
影响因子：
5.6
通讯作者：
D. Ghanotakis
中科院分区：
文献类型：
--
作者：
G. Tsiotis;T. Walz;A. Spyridaki;A. Lustig;A. Engel;D. Ghanotakis
从菠菜中分离出包含所有三种外在蛋白（33、23、17 kDa）的释氧光系统 II 核心复合物，并将其重构为直径 72.9 nm、长度 1-2 微米的管状二维晶体。虽然 17 和 23 kDa 多肽在结晶过程中丢失，但外源 33 kDa 蛋白质被保留。结晶核心的光谱是完整 PSII 核心复合物的特征。免疫电镜显示PSII复合物的管腔表面暴露在圆柱形管的外侧。通过负染色电子显微镜和分辨率为 2.0 nm 的图像分析，从扁平管状晶体中确定复合物的投影。菱形晶胞（a = 16.2 nm，b = 13.7 nm；gamma = 142.4 度）包含一个 PSII 复合物。
An oxygen evolving photosystem II core complex containing all three extrinsic proteins (33, 23, 17 kDa) was isolated from spinach and reconstituted into tubular two-dimensional crystals of 72.9 nm diameter and 1-2 micrometers length. While the 17 and 23 kDa polypeptides were lost during crystallization, the extrinsic 33 kDa protein was retained. The optical spectrum of the crystallized core was characteristic of an intact PSII core complex. Immunoelectron microscopy revealed that the lumenal surface of the PSII complex was exposed at the outside of the cylindrical tubes. The projection of the complex was determined from flattened tubular crystals by negative stain electron microscopy and image analysis to 2.0 nm resolution. Rhombic unit cells (a = 16.2 nm, b = 13.7 nm; gamma = 142.4 degrees) contained one PSII complex.
影响因子：
3
作者：
Lyon,MK;Marr,KM;Furcinitti,PS
通讯作者：
Furcinitti,PS