Refinement of the NMR solution structure of a protein to remove distortions arising from neglect of internal motion.
Refinement of the NMR solution structure of a protein to remove distortions arising from neglect of internal motion.
复制标题
细化蛋白质的 NMR 溶液结构，以消除因忽略内部运动而产生的扭曲。
DOI：
10.1021/bi00230a001
复制
发表时间：
1991
期刊：
影响因子：
2.9
通讯作者：
Markley,JL
中科院分区：
文献类型：
--
作者：
Fejzo,J;Krezel,AM;Westler,WM;Macura,S;Markley,JL
Jasna Fejzo，t，§ Andrzej M. Krezel，§ William M. Westler，5 Slobodan Macura，1，11和John L. Markley 2005年生物化学系，农业和生命科学学院，威斯康星州，420亨利Mall，麦迪逊，威斯康星州53706，和物理化学系，贝尔格莱德大学，11000 Beograd，POB 550，Yugoslavia接收1991年1月29日摘要：实验研究了内部运动对核磁共振交叉弛豫蛋白质结构质量的影响。内部旋转的酪氨酸-31环的火鸡卵类粘蛋白第三域被发现介导的磁化转移，其影响导致低估的质子-质子距离在其附近。区分纯交叉松弛与化学交换介导的交叉松弛的实验方法被用来将真实距离与扭曲距离分开。未校正和校正的距离集，其中校正考虑了内部运动，每个都被用作输入到结构建模的距离几何程序。每一组距离都产生了一系列相似的（收敛的）结构。这两个家族的结构在酪氨酸-31区域有相当大的不同（2A）。此外，差异高达1
Jasna Fejzo, t, § Andrzej M. Krezel, § William M. Westler, 5 Slobodan Macura, 1, 11 and John L. Markley*’5 Biochemistry Department, College of Agricultural and Life Sciences, University of Wisconsin, 420 Henry Mall, Madison, Wisconsin 53706, and Faculty of Physical Chemistry, University of Belgrade, 11000 Beograd, POB 550, Yugoslavia Received January 29, 1991 abstract: The effect of internal motion on the quality of a protein structure derived from nuclear magnetic resonance (NMR) cross relaxation has been investigated experimentally. Internal rotation of the tyrosine-31 ring of turkey ovomucoid third domain was found to mediate magnetization transfer; the effect led to underestimation of proton-proton distances in its immediate neighborhood. Experimental methods that distinguish pure cross relaxation from chemical exchange mediated cross relaxation were used to separate true distances from distorted ones. Uncorrected and corrected sets of distances, where the corrections took internal motion into account, each were used as input to a distance geometry program for structural modeling. Each set of distances yielded a family of similar (converged) structures. The two families of structures differed considerably (2 A) in the region of tyrosine-31. In addition, differences as large as 1