The Tol1 transposable element of the medaka fish moves in human and mouse cells
The Tol1 transposable element of the medaka fish moves in human and mouse cells
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DOI：
10.1007/s10038-007-0161-2
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发表时间：
2007-06-01
影响因子：
3.5
通讯作者：
Hamaguchi, Satoshi
中科院分区：
文献类型：
--
作者：
Koga, Akihiko;Shimada, Atsuko;Hamaguchi, Satoshi
基于 DNA 的转座元件可用作基因工程和基因治疗的工具。与 RNA 介导的元件和逆转录病毒相比，一个巨大的优势是使用简单且安全。青鳉鱼 Oryzias latipes 的 Tol1 元件具有基于 DNA 元件的结构特征。尽管其切除已被证实，但尚未观察到从头插入，并且迄今为止尚未鉴定出转座酶。我们首先通过计算机搜索比对和基因组文库筛选克隆了一个携带开放阅读框的 4.4 kb Tol1 副本，然后通过 mRNA 分析鉴定了编码 851 个氨基酸的 2.9 kb 转录本。该转录物的蛋白质产物催化人和小鼠培养细胞中非自主 Tol1 拷贝的转座。对功能齐全的 Tol1 转座酶的鉴定可能会导致开发用于哺乳动物基础和转化分子生物学应用的新工具。
DNA-based transposable elements can be used as tools for gene engineering and gene therapy. A great advantage over RNA-mediated elements and retroviruses is the simplicity and safety of usage. The Tol1 element of the medaka fish Oryzias latipes has structural features of DNA-based elements. Although its excision has already been demonstrated, de novo insertion has not been observed, and a transposase has not been hitherto identified. We first cloned, through in silico search alignments and genomic library screenings, a 4.4-kb Tol1 copy carrying open reading frames and then identified, by mRNA analysis, a 2.9-kb transcript coding for 851 amino acids. The protein product of this transcript catalyzed transposition of a nonautonomous Tol1 copy in human and mouse culture cells. This identification of a fully functional Tol1 transposase could lead to the development of new tools for basic and translational molecular biology applications in mammals.