Coxiella burnetii isolates originating from infected cattle induce a more pronounced proinflammatory cytokine response compared to isolates from infected goats and sheep
Coxiella burnetii isolates originating from infected cattle induce a more pronounced proinflammatory cytokine response compared to isolates from infected goats and sheep
复制标题
DOI：
10.1093/femspd/ftx040
复制
发表时间：
2017-06-01
影响因子：
3.3
通讯作者：
Rebel, Johanna M.
中科院分区：
文献类型：
--
作者：
Ammerdorffer, Anne;Kuley, Runa;Rebel, Johanna M.
伯氏柯谢氏菌是Q热的病原体。虽然牛的伯纳蒂菌流行率远高于山羊和绵羊，但受感染的牛很少与人类暴发有关。我们研究了人类接触不同宿主来源或不同多位点可变数串联重复分析（MLVA）基因型伯纳蒂胞杆菌分离株后的免疫反应是否不同。用山羊、绵羊、牛、急性和慢性Q热患者的16株已知MLVA基因型伯纳蒂c分离株刺激人外周血单个核细胞（PBMCs），检测细胞因子的反应。与山羊、绵羊和慢性Q热患者的分离株相比，来自牛的分离株诱导的IL-1 β、tnf - α和IL-22明显增加。比较基于MVLA基因型的分离株的细胞因子诱导，未发现MLVA基因型之间的反应差异。来自牛的伯纳蒂胞杆菌分离株在人外周血单核细胞中诱导的促炎细胞因子反应可能解释了牛引起的人Q热暴发的低发病率。慢性Q热患者伯纳蒂胞杆菌分离株刺激PBMCs的细胞因子谱与山羊分离株相似。此外，细胞因子反应似乎取决于宿主来源而不是MLVA基因型。
Coxiella burnetii is the causative agent of Q fever. Although the prevalence of C. burnetii in cattle is much higher than in goats and sheep, infected cattle are rarely associated with human outbreaks. We investigated whether the immune response of humans differs after contact with C. burnetii isolates from different host origins or with different multilocus variable number of tandem repeat analysis (MLVA) genotypes. Cytokine responses were measured in human peripheral blood mononuclear cells (PBMCs) stimulated with 16 C. burnetii isolates with known MLVA genotype from goats, sheep, cattle, acute and chronic Q fever patients. Coxiella burnetii isolates originating from cattle induce significantly more IL-1 beta, TNF-alpha and IL-22 than the isolates from goats, sheep or chronic Q fever patients. Comparing the cytokine induction of the isolates based on their MVLA genotype did not reveal differences in response between the MLVA genotypes. The proinflammatory cytokine response induced in human PBMCs by C. burnetii isolates from cattle may explain the low incidence of human Q fever outbreaks caused by cattle. The cytokine profile of PBMCs stimulated with C. burnetii isolates from chronic Q fever patients resembles isolates from goats. Furthermore, cytokine responses seem to be depending on host origin than on MLVA genotype.