Transcription factor DUO1 generated by neo-functionalization is associated with evolution of sperm differentiation in plants
Transcription factor DUO1 generated by neo-functionalization is associated with evolution of sperm differentiation in plants
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DOI：
10.1038/s41467-018-07728-3
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发表时间：
2018-12-11
影响因子：
16.6
通讯作者：
Araki, Takashi
中科院分区：
文献类型：
--
作者：
Higo, Asuka;Kawashima, Tomokazu;Araki, Takashi
细胞类型创新背后的进化机制在很大程度上仍不清楚。在多细胞真核生物中，精子分化的进化分子起源在大多数谱系中是未知的。在这里，我们报告说，在陆地植物的藻类祖先，MYB转录因子DUO1的祖先的DNA结合结构域的变化，使识别一个新的顺式调节元件。这一事件导致了活动精子的分化。DUO1在新功能化后，在陆地植物的共同祖先中获得精子谱系特异性表达。随后，DUO1的下游网络被重新连接，导致精子具有不同的形态。结合绿色藻类，陆地植物的姐妹群，在DUO1的DNA结合结构域中积累突变并丧失精子分化。我们的研究结果表明，DUO1的出现是陆地植物谱系中精子分化和有性生殖模式多样性进化的决定性事件。
Evolutionary mechanisms underlying innovation of cell types have remained largely unclear. In multicellular eukaryotes, the evolutionary molecular origin of sperm differentiation is unknown in most lineages. Here, we report that in algal ancestors of land plants, changes in the DNA-binding domain of the ancestor of the MYB transcription factor DUO1 enabled the recognition of a new cis-regulatory element. This event led to the differentiation of motile sperm. After neo-functionalization, DUO1 acquired sperm lineage-specific expression in the common ancestor of land plants. Subsequently the downstream network of DUO1 was rewired leading to sperm with distinct morphologies. Conjugating green algae, a sister group of land plants, accumulated mutations in the DNA-binding domain of DUO1 and lost sperm differentiation. Our findings suggest that the emergence of DUO1 was the defining event in the evolution of sperm differentiation and the varied modes of sexual reproduction in the land plant lineage.