MONOCLONAL-ANTIBODY PROPHYLAXIS AGAINST THE INVIVO TOXICITY OF RICIN IN MICE
MONOCLONAL-ANTIBODY PROPHYLAXIS AGAINST THE INVIVO TOXICITY OF RICIN IN MICE
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DOI：
10.3109/08820139309066194
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发表时间：
1993-01-01
影响因子：
2.8
通讯作者：
HEWETSON, JF
中科院分区：
文献类型：
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作者：
CHANH, TC;ROMANOWSKI, MJ;HEWETSON, JF
制备了蓖麻毒素特异性的BALB/c小鼠IgG1单克隆抗体BG11-G2。BG11-G2抗体既不与纯化的蓖麻毒素A链结合，也不与纯化的蓖麻毒素B链结合，但识别出一种抗原决定因子，其构象需要两条链结合才能形成天然蓖麻毒素分子。它不与蛋白质合成抑制剂T-2霉菌毒素或钠通道阻滞剂、蛤蚌毒素和河豚毒素发生反应。通过EL-4细胞[H-3]亮氨酸摄取测定，低至0.78马克杯/毫升的BG11-G2 IgG1抗蓖麻毒素单克隆抗体完全保护了蓖麻毒素的体外毒性。在蓖麻毒素致死性攻击前一天，将纯化的BG11-G2抗体被动腹腔输注BALB/c小鼠，可显著延迟毒性和死亡的发生。蓖麻毒素攻毒前后注入BG11-G2对大鼠有较好的保护作用。
A BALB/c murine IgG1 monoclonal antibody, designated BG11-G2, specific for ricin was generated. BG11-G2 antibody did not bind to either purified ricin chain A or chain B, but recognized an antigenic determinant whose conformation requires the combination of the two chains in the formation of the native ricin molecule. It did not react with the protein synthesis inhibitor, T-2 mycotoxin, or with the sodium channel blockers, saxitoxin and tetrodotoxin. As little as 0.78 mug/ml of BG11-G2 IgG1 anti-ricin monoclonal antibody completely protected against the in vitro toxicity of ricin as determined by [H-3]leucine uptake in EL-4 cell assays. Passive intraperitoneal infusion of purified BG11-G2 antibody into BALB/c mice one day prior to a lethal challenge with ricin considerably delayed the onset of toxicity and death. Better protection was obtained with BG11-G2 infused before and after ricin challenge.