Synthesis of capped RNA using a DMT group as a purification handle.
Synthesis of capped RNA using a DMT group as a purification handle.
复制标题
使用 DMT 基团作为纯化手柄合成加帽 RNA。
DOI：
10.1080/15257770.2013.864417
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发表时间：
2014
期刊：
影响因子：
--
通讯作者：
Jones,RogerA
中科院分区：
文献类型：
--
作者：
Veliath,Elizabeth;Gaffney,BarbaraL;Jones,RogerA
We report a new method for synthesis of capped RNA or 2′-OMe RNA that uses a N2−4,4′-dimethoxytrityl (DMT) group as a lipophilic purification handle to allow convenient isolation and purification of the capped RNA. The DMT group is easily removed under mild conditions without degradation of the cap. We have used this approach to prepare capped 10- and 20-mers. This method is compatible with the many condensation reactions that have been reported for preparation of capped RNA or cap analogues.
