STABILITY OF LIPOSOMES IN-VITRO AND THEIR UPTAKE BY RAT PEYER PATCHES FOLLOWING ORAL-ADMINISTRATION
STABILITY OF LIPOSOMES IN-VITRO AND THEIR UPTAKE BY RAT PEYER PATCHES FOLLOWING ORAL-ADMINISTRATION
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DOI：
10.1023/a:1018936806278
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发表时间：
1993-08-01
影响因子：
3.7
通讯作者：
TSUCHIYA, S
中科院分区：
文献类型：
--
作者：
ARAMAKI, Y;TOMIZAWA, H;TSUCHIYA, S
为了评价脂质体作为将抗原靶向递送至肠相关淋巴组织的载体的有用性，研究了脂质体的稳定性和大鼠派伊尔集合淋巴结的摄取。由二硬脂酰磷脂酰胆碱、磷脂酰丝氨酸和胆固醇（DSPC-脂质体）或二棕榈酰磷脂酰胆碱、磷脂酰丝氨酸和胆固醇组成的脂质体在酸性溶液（pH 2.0）、稀释胆汁和胰酶溶液中稳定。脂质体口服给药后，若丹明B-PE纳入DSPC-脂质体的脂质相优先采取派尔集合淋巴结在回肠下部。平均粒径大于374 nm的DSPC-脂质体对罗丹明B-PE的摄取较高。因此，口服给药的大直径DSPC-脂质体似乎有效地用作将抗原递送至派尔集合淋巴结的载体。
To evaluate the usefulness of liposomes as a carrier for the targeted delivery of antigens to gut-associated lymphoid tissue, liposomal stability and uptake by rat Peyer's patches were investigated. Liposomes composed of distearoylphosphatidylcholine, phosphatidylserine, and cholesterol (DSPC-liposome), or dipalmitoylphosphatidylcholine, phosphatidylserine, and cholesterol were stable in acidic solution (pH 2.0), diluted bile, and pancreatin solution. Following the oral administration of liposomes to rats, rhodamine B-PE incorporated in the lipid phase of DSPC-liposomes was preferentially taken up by Peyer's patches in the lower ileum. The uptake of rhodamine B-PE from DSPC-liposomes larger than 374 nm in mean diameter was high. Orally administered DSPC-liposomes of a large diameter thus appear to serve effectively as a vehicle for delivering antigens to Peyer's patches.