Detection of sialic acids and gangliosides with special reference to 9-O-acetylated species in basaliomas and normal human skin
Detection of sialic acids and gangliosides with special reference to 9-O-acetylated species in basaliomas and normal human skin
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DOI：
10.1046/j.1523-1747.2001.01237.x
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发表时间：
2001-02-01
影响因子：
6.5
通讯作者：
Schauer, R
中科院分区：
文献类型：
--
作者：
Fahr, C;Schauer, R
检查基底细胞癌和正常皮肤与神经节苷脂异常表达的关系。通过使用丙型流感病毒的微量滴定测定以及释放的唾液酸的荧光高效液相色谱法分析了 26 个样品对中具有 9-O-乙酰化唾液酸的神经节苷脂的含量。基底细胞癌组织中的 9-O-乙酰化水平显着高于基底细胞癌周围皮肤的水平（高达 56 倍）。在基底瘤边缘的皮肤中也发现 O-乙酰化神经节苷脂含量略有升高。使用针对 9-O-乙酰 GD3 的单克隆抗体，通过高效薄层色谱和免疫高效薄层色谱研究了四组基底细胞癌合并样本和一组正常皮肤的神经节苷脂组成。正常皮肤的脂质结合唾液酸含量为 0.029 杯干重，而结节性基底细胞癌的含量约为其两倍。高效液相色谱分析发现多种O-乙酰化唾液酸，但主要为N-乙酰基-9-O-乙酰神经氨酸。仅在肿瘤神经节苷脂部分中，发现了少量的N-乙醇酰神经氨酸。以9-O-乙酰基-GD3为主的9-O-乙酰化神经节苷脂可被认为是肿瘤相关抗原或基底细胞的标记物。癌。关于肿瘤相关碳水化合物的这一发现可能有助于当前肿瘤诊断和治疗的新策略。
Basal cell carcinomas and normal skin were examined in relation to the abnormal expression of gangliosides. The content of gangliosides with 9-O-acetylated sialic acids of 26 sample pairs was analyzed by a microtiter assay using influenza C virus as well as by fluorimetric high-performance liquid chromatography of the sialic acids released. The 9-O-acetylation levels were significantly (up to 56-fold) higher in basal cell carcinoma tissues than in the skin surrounding basal cell carcinomas. Slightly elevated amounts of O-acetylated gangliosides were also seen in the skin marginal to the basaliomas. The ganglioside composition of four sets of pooled samples of basal cell carcinoma and one pool of normal skin were studied by high-performance thin-layer chromatography and immune high-performance thin-layer chromatography using monoclonal antibodies against 9-O-acetyl GD3. The lipid-bound sialic acid content of normal skin was 0.029 mug dry weight, whereas in nodular basal cell carcinomas it was approximately twice as much. Several O-acetylated sialic acids were seen by high-performance liquid chromatography analysis, but N-acetyl-9-O-acetylneuraminic acid prevailed. Only in the tumor ganglioside fraction, a small amount of N-glycolylneuraminic acid was found. The 9-O-acetylated gangliosides with mainly 9-O-acetyl-GD3 can be considered as tumor-associated antigens or markers for basal cell. carcinomas. This finding about tumor-associated carbohydrates may contribute to new strategies in current tumor diagnosis and therapy.