Isolation and characterization of two glycoproteins from patients with alveolar proteinosis.
Isolation and characterization of two glycoproteins from patients with alveolar proteinosis.
复制标题
肺泡蛋白沉积症患者两种糖蛋白的分离和表征。
DOI：
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发表时间：
1973
影响因子：
11.1
通讯作者：
W. Lynn
中科院分区：
文献类型：
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作者：
M. Passero;R. Tye;K. Kilburn;W. Lynn
从4例肺泡蛋白沉积症患者的肺灌洗中获得的盐水不溶性颗粒物质分析了总脂质（52%）、蛋白质（44%）和碳水化合物（4%）。十二烷基硫酸钠凝胶电泳显示，只有三个主要的肽在这些患者。分子量（来自凝胶）为69，000、62，000和36，000，后两种肽为高碘酸-希夫染色阳性。氨基酸和糖的分析进行了从凝胶切割的肽和通过Sephadex G-200色谱纯化的肽。69，000分子量的肽不含碳水化合物。抗体研究和氨基酸分析表明它是白蛋白。分子量为62，000的肽含有1%的羟脯氨酸，1%的羟赖氨酸，10%的甘氨酸和9%的碳水化合物。36，000分子量的肽含有1.2%羟脯氨酸、1%羟赖氨酸、13%甘氨酸、1.4%唾液酸、2.6%葡萄糖、2.4%半乳糖、2%甘露糖、0.8%岩藻糖、0.4%葡糖胺和0.6%半乳糖胺。先前已经观察到从肾小球中提取的具有相似氨基酸和碳水化合物组成的蛋白质。
The saline-insoluble particulate material obtained by pulmonary lavage from four patients with alveolar proteinosis was analyzed for total lipid (52%), protein (44%), and carbohydrate (4%). Sodium dodecyl sulfate-gel electrophoresis revealed only three major peptides in these patients. Molecular weights (from the gels) were 69,000, 62,000, and 36,000, and the latter two peptides were periodic acid-Schiff stain-positive. Amino-acid and sugar analyses were performed on the peptides cut from the gels and on peptides purified by Sephadex G-200 chromatography. The 69,000-molecular-weight peptide contained no carbohydrate. Antibody studies and aminoacid analysis indicated that it was albumin. The 62,000-molecular-weight peptide contained 1% hydroxyproline, 1% hydroxylysine, 10% glycine, and 9% carbohydrate. The 36,000-molecular-weight peptide contained 1.2% hydroxyproline, 1% hydroxylysine, 13% glycine, 1.4% sialic acid, 2.6% glucose, 2.4% galactose, 2% mannose, 0.8% fucose, 0.4% glycosamine, and 0.6% galactosamine. Proteins extracted from kidney glomeruli with similar amino-acid and carbohydrate composition have been observed previously.