PROTECTION OF GOATS AGAINST PESTE DES PETITS RUMINANTS WITH A VACCINIA VIRUS DOUBLE RECOMBINANT EXPRESSING THE F-GENE AND H-GENE OF RINDERPEST VIRUS
PROTECTION OF GOATS AGAINST PESTE DES PETITS RUMINANTS WITH A VACCINIA VIRUS DOUBLE RECOMBINANT EXPRESSING THE F-GENE AND H-GENE OF RINDERPEST VIRUS
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DOI：
10.1016/0264-410x(93)90386-c
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发表时间：
1993-07-01
期刊：
影响因子：
5.5
通讯作者：
YILMA, T
中科院分区：
文献类型：
--
作者：
JONES, L;GIAVEDONI, L;YILMA, T
小反刍兽疫（PPR）是山羊和绵羊的一种病毒性疾病，以坏死性和糜烂性口腔炎、肠炎和肺炎为特征。病原体PPRV是副粘病毒科和麻疹病毒属的成员。该属的其他成员包括牛瘟病毒（RPV）、麻疹病毒、犬瘟热病毒和福西德犬瘟热病毒。PPR具有非常高的发病率和死亡率，有效控制这种疾病在非洲、亚洲和中东具有重要的经济意义。目前还没有安全有效的疫苗来预防这种疾病。组织培养牛瘟疫苗（TCRV）可保护小型反刍动物免受严重疾病的侵害;然而，存在与疫苗接种相关的临床问题。本实验室最近研制了几种有效的牛瘟重组痘苗病毒疫苗。这些疫苗易于接种，生产成本低，热稳定。用表达RPV血凝素和融合基因的痘苗病毒双重组体免疫山羊。尽管接种疫苗的动物产生了对RPV的抗体（中和和ELISA），而不是对PPRV的抗体，但它们对强毒PPRV的攻毒接种具有完全的保护作用。这将表明保护最有可能是由于细胞介导的免疫。在世界上PPRV流行的地区使用牛瘟双重重组牛痘病毒将有助于控制和根除PPR。
Peste des petits ruminants (PPR) is a viral disease of goats and sheep characterized by necrotizing and erosive stomatitis, enteritis and pneumonia. The causative agent, PPRV, is a member of the family Paramyxoviridae and the genus Morbillivirus. Other members of the genus include rinderpest (RPV), measles, canine distemper and phocid distemper viruses. PPR has a very high rate of morbidity and mortality, and effective control of this disease is of economic importance in Africa, Asia and the Middle East. Currently, there is no safe and effective vaccine available against the disease. The tissue culture rinderpest vaccine (TCRV) protects small ruminants against severe disease; there are, however, clinical problems associated with vaccination. This laboratory has recently developed several effective vaccinia virus recombinant vaccines for rinderpest. These vaccines are easy to administer, inexpensive to produce and heat-stable. Goats were vaccinated with a vaccinia virus double recombinant expressing the haemagglutinin and fusion genes of RPV. Although vaccinated animals developed antibodies (neutralizing and ELISA) to RPV, and not to PPRV, they were completely protected against challenge inoculation with virulent PPRV. This would indicate that protection is most probably due to cell-mediated immunity. Use of the rinderpest double recombinant vaccinia virus in areas of the world where PPRV is endemic would aid in the control and eradication of PPR.