Experimental inoculation study indicates swine as a potential host for Hendra virus
Experimental inoculation study indicates swine as a potential host for Hendra virus
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DOI：
10.1051/vetres/2010005
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发表时间：
2010-05-01
影响因子：
4.4
通讯作者：
Weingartl, Hana M.
中科院分区：
文献类型：
--
作者：
Li, Mingyi;Embury-Hyatt, Carissa;Weingartl, Hana M.
亨德拉病毒(HEV)是一种来自副粘病毒科的人畜共患病毒，可导致人和马的致命疾病。五周大的长白猪和五个月大的哥廷根小型猪每只动物接种大约10(7)个菌斑形成单位。除了所有感染的猪都表现出发烧和抑郁外，两头长白猪中的一头在接种后5天出现呼吸道症状，其中一头哥廷根小型猪在接种后5天出现呼吸体征，在7天出现轻微的神经体征。针对HEV M基因的实时RT-PCR检测结果显示，所有感染猪在2-5dpi的口腔、鼻腔和直肠拭子和3-5dpi的眼拭子中均可检测到病毒。鼻拭子中病毒滴度高达10(4.6)TCID50/毫升。病毒RNA在感染早期主要分布在呼吸道和淋巴系统的组织中，病毒分离证实病毒的存在。病毒抗原的病理改变和免疫组织化学染色与病毒的组织分布一致。这一新发现表明，猪对HEV感染很敏感，并可能在向人类传播的过程中发挥中间宿主的作用。
Hendra virus (HeV) is a zoonotic virus from the family Paramyxoviridae causing fatal disease in humans and horses. Five-week-old Landrace pigs and 5-month-old Gottingen minipigs were inoculated with approximately 10(7) plaque forming units per animal. In addition to fever and depression exhibited in all infected pigs, one of the two Landrace pigs developed respiratory signs at 5 days post-inoculation (dpi) and one of the Gottingen minipigs developed respiratory signs at 5 dpi and mild neurological signs at 7 dpi. Virus was detected in all infected pigs at 2-5 dpi from oral, nasal, and rectal swabs and at 3-5 dpi from ocular swabs by real-time RT-PCR targeting the HeV M gene. Virus titers in nasal swab samples were as high as 10(4.6) TCID50/mL. The viral RNA was mainly distributed in tissues from respiratory and lymphoid systems at an early stage of infection and the presence of virus was confirmed by virus isolation. Pathological changes and immunohistochemical staining for viral antigen were consistent with the tissue distribution of the virus. This new finding indicates that pigs are susceptible to HeV infections and could potentially play a role as an intermediate host in transmission to humans.