Expression of cellular FLICE/caspase-8 inhibitory protein is associated with malignant potential in endometrial carcinoma
Expression of cellular FLICE/caspase-8 inhibitory protein is associated with malignant potential in endometrial carcinoma
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DOI：
10.1111/j.1525-1438.2005.00122.x
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发表时间：
2005-07-01
影响因子：
4.8
通讯作者：
Luo, RY
中科院分区：
文献类型：
--
作者：
Chen, HX;Liu, YJ;Luo, RY
本研究旨在探讨细胞Fas相关死亡域样白介素1β转换酶(FLICE)/半胱氨酸天冬氨酸氨基转移酶-8抑制蛋白(c-FliP)在子宫内膜癌中的表达及其可能的意义。应用免疫组织化学方法检测42例子宫内膜癌组织和22例正常增生期子宫内膜组织中C-Flip蛋白的表达。用SYBR Green I-TM半定量逆转录聚合酶链式反应(RT-PCR)检测20例子宫内膜癌和18例正常增生期子宫内膜中c-flip信使核糖核酸(MRNA)的表达。分析c-Flip蛋白水平与子宫内膜癌患者肿瘤细胞增殖及临床病理参数的关系。C-flip蛋白在肿瘤组织中的表达显著高于正常组织(P<；0.01)，RT-PCR检测c-flip mRNA的结果与此相似(P<；0.01)。此外，c-flip蛋白与增殖细胞核抗原标记指数(P<；0.01)、临床分期(P<；0.05)、1/2肌层侵犯(P<；0.05)、淋巴结转移(P<；0.01)显著相关。多变量分析也证实c-FLIP与临床分期(P<；0.05)和淋巴结转移(P<；0.05)有关，而与肌层侵袭关系不大(P=0.059)。结论：c-FLIP在子宫内膜癌的发生、发展中起重要作用，可作为判断子宫内膜癌预后的有用指标。
This study aimed to investigate the expression of cellular Fas-associated death domain-like interleukin-1 beta-converting enzyme (FLICE)/caspase-8 inhibitory protein (c-FLIP) in endometrial carcinoma and its possible implications. c-FLIP protein was detected in 42 endometrial carcinoma tissues and in 22 normal proliferative endometrial tissues by immunohistochemistry. In addition, c-FLIP messenger ribonucleic acid (mRNA) was evaluated in 20 endometrial carcinomas and in 18 normal proliferative endometria by semiquantitative reverse transcriptase-polymerase chain reaction (RT-PCR) using SYBR Green I-TM. The relationship between c-FLIP protein level and tumor cell proliferation and that between c-FLIP protein level and clinicopathologic parameters of patients with endometrial carcinoma was analyzed. c-FLIP protein expression was significantly higher in neoplastic tissues than in normal tissues (P < 0.01), and similar result was obtained from RT-PCR analysis of c-FLIP mRNA (P < 0.01). Furthermore, c-FLIP protein was significantly associated with proliferating cell nuclear antigen-labeling index (P < 0.01), clinical stage (P < 0.05), the presence of invasion to > 1/2 myometrium (P < 0.05), and lymph node metastasis (P < 0.01). Multivariate analysis of variance also confirmed the association of c-FLIP with clinical stage (P < 0.05) and with lymph node metastasis (P < 0.05), while its association with myometrial invasion was marginal (P= 0.059). It is concluded that c-FLIP might contribute to the carcinogenesis and aggressiveness of endometrial carcinoma and might be a useful prognostic factor in the tumor.