Transplantation of marrow-derived mesenchymal stem cells and platelet-rich plasma during distraction osteogenesis - a preliminary result of three cases
Transplantation of marrow-derived mesenchymal stem cells and platelet-rich plasma during distraction osteogenesis - a preliminary result of three cases
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DOI：
10.1016/j.bone.2004.06.013
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发表时间：
2004-10-01
期刊：
影响因子：
4.1
通讯作者：
Ishiguro, N
中科院分区：
文献类型：
--
作者：
Kitoh, H;Kitakoji, T;Ishiguro, N
回顾了2例软骨发育不全患者和1例先天性胫骨假关节患者的3个股骨和2个胫骨移植骨髓间充质干细胞（MSCs）和富血小板血浆（PRP）牵引成骨的临床结果。来源于髂嵴的MSC与成骨补充剂一起培养并分化为成骨样细胞。PRP，已知含有几种生长因子，并通过一分钟的凝血酶和钙的引入立即凝固，在移植前制备。将培养扩增的成骨细胞样细胞和自体PRP与凝血酶-钙混合物一起注射到牵引的骨痂中，以便PRP凝胶可以在注射部位内形成。在每例患者的延长和巩固期进行MSCs和PRP移植。每条腿均达到目标长度，无严重并发症，平均愈合指数为23.0天/cm（18.8-26.9天/cm）。虽然这些结果仍然是初步的，成骨细胞样细胞和PRP的移植，这似乎是一个安全和微创的细胞治疗，可以缩短治疗时间，加速骨再生过程中牵张成骨。(C)2004年爱思唯尔公司All rights reserved.
Clinical results of distraction osteogenesis with transplantation of marrow-derived mesenchymal stem cells (MSCs) and platelet-rich plasma (PRP) were reviewed in three femora and two tibiae of the two patients with achondroplasia and one patient with congenital pseudarthrosis of the tibia. MSCs derived from the iliac crest were cultured with osteogenic supplements and differentiated into osteoblast-like cells. PRP, which is known to contain several growth factors and coagulate immediately by a minute introduction of thrombin and calcium, was prepared just before transplantation. Culture-expanded osteoblast-like cells and autologous PRP were injected into the distracted callus with the thrombin-calcium mixture so that the PRP gel might develop within the injected site. Transplantation of MSCs and PRP was done at the lengthening and consolidation period in each patient. The target lengths were obtained in every leg without major complications and the average healing index was 23.0 days/cm (18.8-26.9 days/cm). Although these results are still preliminary, transplantation of osteoblast-like cells and PRP, which seemed to be a safe and minimally invasive cell therapy, could shorten the treatment period by acceleration of bone regeneration during distraction osteogenesis. (C) 2004 Elsevier Inc. All rights reserved.