Characterization of Cu and Cd biosorption by Pseudomonas sp. strain DC-B3 isolated from metal mine soil
Characterization of Cu and Cd biosorption by Pseudomonas sp. strain DC-B3 isolated from metal mine soil
复制标题
假单胞菌对铜和镉生物吸附的表征。
DOI：
10.1007/s13762-018-2011-5
复制
发表时间：
2018-09
影响因子：
3.1
通讯作者：
Yin B Y
中科院分区：
文献类型：
--
作者：
Liang Y;Chen J Q;Mei J;Chang J J;Wang Q Y;Wan G S;Yin B Y
从某铜矿区重金属污染土壤中分离到一株耐Cu、Cd的细菌DC-B3，经鉴定属于假单胞菌属（Pseudomonassp.），并能从水溶液中吸附Cu ~（2+）和Cd ~（2+）。通过批次实验研究了其作为环境参数（如接触时间、初始浓度、pH、温度和细菌剂量）的函数的生物吸附性能，并且在pH 6.0、40 °C和0.5 g生物量L−1、初始浓度为105 mg L− 1时去除能力达到峰值。吸附发生非常迅速，在开始时，达到平衡后，约1小时和4小时的Cu 2+和Cd 2+，分别和两种金属的生物吸附动力学数据拟合良好的伪二级模型（r2 0.99）。吸附等温线表明，这两种金属的吸附是非常有利的，平衡数据更好地描述了Langmuir模型（r2 0.98为Cu 2+和0.99为Cd 2+），与估计的最大吸附容量为12.6和26.5毫克g−1的Cu 2+和Cd 2+，分别。解吸试验表明，生物吸附是一个化学过程，一部分金属离子可能进入细菌细胞。傅里叶变换红外光谱分析表明，细菌细胞壁上的羟基、氨基、羧基和磷酸根官能团可能是吸附的主要原因，但它们对Cu ~（2+）和Cd ~（2+）的吸附贡献不同。这一结果表明，菌株DC-B3可以作为一个潜在的生态友好和有效的生物吸附剂修复铜（II）和镉（II）污染物。
A Cu- and Cd-resistant bacterial strain, DC-B3, was isolated from heavy metal-contaminated soils in a Cu mine, was identified as belonging to Pseudomonas sp. and biosorbed Cu2+ and Cd2+ from aqueous solution in the present study. Its biosorption properties as a function of environmental parameters such as contact time, initial concentration, pH, temperature and bacterial dosage were investigated through batch experiments, and the removal capacity peaked at pH 6.0, 40 °C and 0.5 g biomass L−1 at an initial concentration of 105 mg L−1. Sorption occurred extremely rapidly at the beginning, equilibrium was reached after approximately 1 h and 4 h for Cu2+ and Cd2+, respectively, and the biosorption kinetic data for both metals were well fitted by the pseudo-second-order model (r2 > 0.99). Sorption isotherms showed that the adsorptions of both metals were highly favourable, and equilibrium data were better described by the Langmuir model (r2 > 0.98 for Cu2+ and 0.99 for Cd2+), with the estimated maximum sorption capacities of 12.6 and 26.5 mg g−1 for Cu2+ and Cd2+, respectively. Desorption assays suggested that biosorption was a chemical process and that a fraction of the metal ions might have entered the bacterial cells. Fourier transform infrared spectroscopy analysis revealed that hydroxyl, amino, carboxyl and phosphate functional groups on the bacterial cell walls might be mainly responsible for the biosorption, although their contributions to the binding of Cu2+ and Cd2+ were different. This result implied that the strain DC-B3 can be used as a potentially eco-friendly and efficient biosorbent for the remediation of Cu(II) and Cd(II) contaminants.
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DOI：
10.1016/j.saa.2004.03.029
发表时间：
2005-02-01
影响因子：
4.4
作者：
Lin, ZY;Wu, JM;Yang, Y
通讯作者：
Yang, Y
影响因子：
11.4
作者：
Kula, Ibrahim;Ugurlu, Mehmet;Celik, Ali
通讯作者：
Celik, Ali
影响因子：
13.6
作者：
A. Ozer;D. Ozer
通讯作者：
A. Ozer;D. Ozer
影响因子：
5.8
作者：
J. Park;H. Chon
通讯作者：
J. Park;H. Chon
影响因子：
11.4
作者：
N. Yee;L. Benning;V. Phoenix;F. Ferris
通讯作者：
N. Yee;L. Benning;V. Phoenix;F. Ferris